Covalent capture of phospho-dependent protein oligomerization by site-specific incorporation of a diazirine photo-cross-linker

Miquel Vila-Perellö, Matthew R. Pratt, Frej Tulin, Tom W. Muir

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

In this paper, we describe the efficient synthesis of a diazirine cross-linker (photo-Met) and demonstrate that this amino acid is compatible with solid-phase peptide synthesis and expressed protein ligation (EPL). Accordingly, we have exploited the unique power of semi-synthesis to incorporate multiple different probes into the same protein by preparing a version of Smad2 signaling protein containing both photo-Met and an activating phospho-serine. Through judicious placement of the cross-linker, we have been able to covalently capture a transient MH2-MH2 interaction that is dependent upon the PTM in the protein.

Original languageEnglish (US)
Pages (from-to)8068-8069
Number of pages2
JournalJournal of the American Chemical Society
Volume129
Issue number26
DOIs
StatePublished - Jul 4 2007
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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