Current Protocols is issuing corrections for the following Protocol article. Maturana, C. J., Verpeut, J. L., & Engel, E. A. (2022). Single-cell quantification of triple-AAV vector genomes coexpressed in neurons. Current Protocols, 2, e430. doi: 10.1002/cpz1.430 In the above-referenced article: Basic Protocol 1, steps 1 to 3: The instructions for splitting the cells have been clarified, and some redundancy has been removed. Basic Protocol 1, step 16: “3000 rpm” has been replaced with the correct relative centrifugal force value. Basic Protocol 1, step 23: the Optiseal tube has been replaced with a “35-mm PA Ultracrimp tube.” Basic Protocol 1, step 30: The centrifugation time has been corrected from 30 min to 3 min. Basic Protocol 2, step 3, and the Figure 2 legend: Poly-D-ornithine has been corrected to “poly-DL-ornithine” to match the materials list entry. Basic Protocol 4, step 5, substep “c”: The reference to “deep red fluorescence” has been removed. Figure 7 has been replaced with an updated version which now includes the panel identifiers A, B, C, D. The current version online now includes these corrections and may be considered the authoritative version of record.
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)
- Immunology and Microbiology(all)
- Pharmacology, Toxicology and Pharmaceutics(all)
- Health Informatics
- Medical Laboratory Technology