Control of the type 3 secretion system in Vibrio harveyi by quorum sensing through repression of exsA

Christopher M. Waters, Julie T. Wu, Meghan E. Ramsey, Rebecca C. Harris, Bonnie Lynn Bassler

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

The type 3 secretion system (T3SS) genes of Vibrio harveyi are activated at low cell density and repressed at high cell density by quorum sensing (QS). Repression requires LuxR, the master transcriptional regulator of QS-controlled genes. Here, we determine the mechanism underlying the LuxR repression of the T3SS system. Using a fluorescence-based cell sorting approach, we isolated V. harveyi mutants that are unable to express T3SS genes at low cell density and identified two mutations in the V. harveyi exsBA operon. While LuxR directly represses the expression of exsBA, complementation and epistasis analyses reveal that it is the repression of exsA expression, but not exsB expression, that is responsible for the QS-mediated repression of T3SS genes at high cell density. The present work further defines the genes in the V. harveyi QS regulon and elucidates a mechanism demonstrating how multiple regulators can be linked in series to direct the expression of QS target genes specifically at low or high cell density.

Original languageEnglish (US)
Pages (from-to)4996-5004
Number of pages9
JournalApplied and Environmental Microbiology
Volume76
Issue number15
DOIs
StatePublished - Aug 2010

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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