TY - JOUR
T1 - Control of the mitotic cleavage plane by local epithelial topology
AU - Gibson, William T.
AU - Veldhuis, James H.
AU - Rubinstein, Boris
AU - Cartwright, Heather N.
AU - Perrimon, Norbert
AU - Brodland, G. Wayne
AU - Nagpal, Radhika
AU - Gibson, Matthew C.
N1 - Funding Information:
The authors gratefully acknowledge support for this research from the Stowers Institute for Medical Research and the Burroughs Wellcome Fund (to M.C.G.), from the National Science Foundation (to R.N.), from the Natural Sciences and Engineering Research Council of Canada (to G.W.B.), and from the Howard Hughes Medical Institute (to N.P.). W.T.G. was supported in part by NIH/NIGMS Molecular Biophysics Training Grant #T32 GM008313 and CTC grant 1029 to M.C.G.
PY - 2011/2/4
Y1 - 2011/2/4
N2 - For nearly 150 years, it has been recognized that cell shape strongly influences the orientation of the mitotic cleavage plane (e.g.; Hofmeister, 1863). However, we still understand little about the complex interplay between cell shape and cleavage-plane orientation in epithelia, where polygonal cell geometries emerge from multiple factors, including cell packing, cell growth, and cell division itself. Here, using mechanical simulations, we show that the polygonal shapes of individual cells can systematically bias the long-axis orientations of their adjacent mitotic neighbors. Strikingly, analyses of both animal epithelia and plant epidermis confirm a robust and nearly identical correlation between local cell topology and cleavage-plane orientation in vivo. Using simple mathematics, we show that this effect derives from fundamental packing constraints. Our results suggest that local epithelial topology is a key determinant of cleavage-plane orientation, and that cleavage-plane bias may be a widespread property of polygonal cell sheets in plants and animals.
AB - For nearly 150 years, it has been recognized that cell shape strongly influences the orientation of the mitotic cleavage plane (e.g.; Hofmeister, 1863). However, we still understand little about the complex interplay between cell shape and cleavage-plane orientation in epithelia, where polygonal cell geometries emerge from multiple factors, including cell packing, cell growth, and cell division itself. Here, using mechanical simulations, we show that the polygonal shapes of individual cells can systematically bias the long-axis orientations of their adjacent mitotic neighbors. Strikingly, analyses of both animal epithelia and plant epidermis confirm a robust and nearly identical correlation between local cell topology and cleavage-plane orientation in vivo. Using simple mathematics, we show that this effect derives from fundamental packing constraints. Our results suggest that local epithelial topology is a key determinant of cleavage-plane orientation, and that cleavage-plane bias may be a widespread property of polygonal cell sheets in plants and animals.
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U2 - 10.1016/j.cell.2010.12.035
DO - 10.1016/j.cell.2010.12.035
M3 - Article
C2 - 21295702
AN - SCOPUS:79551670667
SN - 0092-8674
VL - 144
SP - 427
EP - 438
JO - Cell
JF - Cell
IS - 3
ER -