Continuous microfluidic immunomagnetic cell separation

David W. Inglis; R. Riehn; R. H. Austin; J. C. Sturm

doi:10.1063/1.1823015

Continuous microfluidic immunomagnetic cell separation

David W. Inglis
, R. Riehn
, R. H. Austin
, J. C. Sturm

Research output: Contribution to journal › Article › peer-review

338 Scopus citations

Abstract

We present a continuous-flow microfluidic device that enables cell by cell separation of cells selectively tagged with magnetic nanoparticles. The cells flow over an array of microfabricated magnetic stripes, which create a series of high magnetic field gradients that trap the magnetically labeled cells and alter their flow direction. The process was observed in real time using a low power microscope. The device has been demonstrated by the separation of leukocytes from whole human blood.

Original language	English (US)
Pages (from-to)	5093-5095
Number of pages	3
Journal	Applied Physics Letters
Volume	85
Issue number	21
DOIs	https://doi.org/10.1063/1.1823015
State	Published - Nov 2004

All Science Journal Classification (ASJC) codes

Physics and Astronomy (miscellaneous)

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10.1063/1.1823015

Cite this

@article{530e75fe5d4847d9956c1052e0ed0633,

title = "Continuous microfluidic immunomagnetic cell separation",

abstract = "We present a continuous-flow microfluidic device that enables cell by cell separation of cells selectively tagged with magnetic nanoparticles. The cells flow over an array of microfabricated magnetic stripes, which create a series of high magnetic field gradients that trap the magnetically labeled cells and alter their flow direction. The process was observed in real time using a low power microscope. The device has been demonstrated by the separation of leukocytes from whole human blood.",

author = "Inglis, \{David W.\} and R. Riehn and Austin, \{R. H.\} and Sturm, \{J. C.\}",

note = "Funding Information: The authors greatly appreciate the many helpful conversations with B. Frazier, L. R. Huang, C. Prinz, S. Park and W. Ryu, and the previous work of M. Berger and M. Shaw. They also thank N. P. Ong and W. L. Lee for their assistance with the magnetometer. This work was supported by grants from DARPA (MDA972-00-1-0031), NIH (HG01506, E–21–F46–G1), NSF Nanobiology Technology Center (BSCECS9876771), the State of New Jersey (NJCST 99-100-082-2042-007), and the Office of Naval Research (ONR3396044). The authors acknowledge support from the Cornell University National Fabrication Center, where the chemical mechanical polishing and deep silicon etching were performed. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

year = "2004",

month = nov,

doi = "10.1063/1.1823015",

language = "English (US)",

volume = "85",

pages = "5093--5095",

journal = "Applied Physics Letters",

issn = "0003-6951",

publisher = "American Institute of Physics",

number = "21",

}

TY - JOUR

T1 - Continuous microfluidic immunomagnetic cell separation

AU - Inglis, David W.

AU - Riehn, R.

AU - Austin, R. H.

AU - Sturm, J. C.

N1 - Funding Information: The authors greatly appreciate the many helpful conversations with B. Frazier, L. R. Huang, C. Prinz, S. Park and W. Ryu, and the previous work of M. Berger and M. Shaw. They also thank N. P. Ong and W. L. Lee for their assistance with the magnetometer. This work was supported by grants from DARPA (MDA972-00-1-0031), NIH (HG01506, E–21–F46–G1), NSF Nanobiology Technology Center (BSCECS9876771), the State of New Jersey (NJCST 99-100-082-2042-007), and the Office of Naval Research (ONR3396044). The authors acknowledge support from the Cornell University National Fabrication Center, where the chemical mechanical polishing and deep silicon etching were performed. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

PY - 2004/11

Y1 - 2004/11

N2 - We present a continuous-flow microfluidic device that enables cell by cell separation of cells selectively tagged with magnetic nanoparticles. The cells flow over an array of microfabricated magnetic stripes, which create a series of high magnetic field gradients that trap the magnetically labeled cells and alter their flow direction. The process was observed in real time using a low power microscope. The device has been demonstrated by the separation of leukocytes from whole human blood.

AB - We present a continuous-flow microfluidic device that enables cell by cell separation of cells selectively tagged with magnetic nanoparticles. The cells flow over an array of microfabricated magnetic stripes, which create a series of high magnetic field gradients that trap the magnetically labeled cells and alter their flow direction. The process was observed in real time using a low power microscope. The device has been demonstrated by the separation of leukocytes from whole human blood.

UR - https://www.scopus.com/pages/publications/19144368234

UR - https://www.scopus.com/pages/publications/19144368234#tab=citedBy

U2 - 10.1063/1.1823015

DO - 10.1063/1.1823015

M3 - Article

AN - SCOPUS:19144368234

SN - 0003-6951

VL - 85

SP - 5093

EP - 5095

JO - Applied Physics Letters

JF - Applied Physics Letters

IS - 21

ER -

Continuous microfluidic immunomagnetic cell separation

Abstract

All Science Journal Classification (ASJC) codes

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