TY - JOUR
T1 - Construction of a viable SV40 variant containing two functional origins of DNA replication
AU - Shenk, Thomas
N1 - Funding Information:
I acknowledge the competent technical assistance of Ms. Bonnie Massey and Mrs. Bridget Prindle. This work was supported by a USPHS research grant from the National Cancer Institute. This work was carried out during the tenure of an Established Investi-gatorship of the American Heart Association.
PY - 1978/4
Y1 - 1978/4
N2 - Viable variants of simian virus 40 (SV40) have been constructed which contain two functional origins of DNA replication (Or). The variants were made by introducing, at 0.175 on the SV40 map, a segment of DNA containing the viral Or. Two types of experiments demonstrate that the second Or is functional. First, the distribution of radioactivity in pulse-labeled SV40 (I) DNA is dramatically altered in the variants when compared with the parental virus. Second, electron microscopic examination of viral replicative intermediates indicates that while there is one initiation site for DNA synthesis in the parental genome, there are two sites in the variant. It was possible to introduce a deletion which inactivated the original Or at 0.67 map units in this variant. The resulting mutant could be propagated, and its DNA replication originated at the site of the newly inserted Or.
AB - Viable variants of simian virus 40 (SV40) have been constructed which contain two functional origins of DNA replication (Or). The variants were made by introducing, at 0.175 on the SV40 map, a segment of DNA containing the viral Or. Two types of experiments demonstrate that the second Or is functional. First, the distribution of radioactivity in pulse-labeled SV40 (I) DNA is dramatically altered in the variants when compared with the parental virus. Second, electron microscopic examination of viral replicative intermediates indicates that while there is one initiation site for DNA synthesis in the parental genome, there are two sites in the variant. It was possible to introduce a deletion which inactivated the original Or at 0.67 map units in this variant. The resulting mutant could be propagated, and its DNA replication originated at the site of the newly inserted Or.
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U2 - 10.1016/0092-8674(78)90229-5
DO - 10.1016/0092-8674(78)90229-5
M3 - Article
C2 - 207442
AN - SCOPUS:0017880213
SN - 0092-8674
VL - 13
SP - 791
EP - 798
JO - Cell
JF - Cell
IS - 4
ER -