Construction and expression in vivo of an internally deleted mouse α-fetoprotein gene: Presence of a transcribed alu-like repeat within the first intervening sequence

Peter R. Young; Richard W. Scott; Dean H. Hamer; Shirley M. Tilghman

doi:10.1093/nar/10.10.3099

Construction and expression in vivo of an internally deleted mouse α-fetoprotein gene: Presence of a transcribed alu-like repeat within the first intervening sequence

Peter R. Young, Richard W. Scott, Dean H. Hamer, Shirley M. Tilghman

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Abstract

An α-fetoprotein (AFP)^* 'minigene' was constructed by joining the first two exons along with 0.9 kilobases of 5′ flanking sequence of the mouse AFP gene to its last exon and 0.4 kilobases of 3′ flanking sequence. This 'minigene' was tested for activity by inserting it into an SV40 vector and infecting African green monkey kidney cells. Correct initiation, termination, polyadenylation and splicing were observed. Furthermore a 220 nucleotide transcript was detected and mapped to a mouse Alu-like or Bl repeat on the opposite strand to that encoding the AFP gene in the first intervening sequence.

Original language	English (US)
Pages (from-to)	3099-3116
Number of pages	18
Journal	Nucleic acids research
Volume	10
Issue number	10
DOIs	https://doi.org/10.1093/nar/10.10.3099
State	Published - May 25 1982

All Science Journal Classification (ASJC) codes

Genetics

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title = "Construction and expression in vivo of an internally deleted mouse α-fetoprotein gene: Presence of a transcribed alu-like repeat within the first intervening sequence",

abstract = "An α-fetoprotein (AFP)* 'minigene' was constructed by joining the first two exons along with 0.9 kilobases of 5′ flanking sequence of the mouse AFP gene to its last exon and 0.4 kilobases of 3′ flanking sequence. This 'minigene' was tested for activity by inserting it into an SV40 vector and infecting African green monkey kidney cells. Correct initiation, termination, polyadenylation and splicing were observed. Furthermore a 220 nucleotide transcript was detected and mapped to a mouse Alu-like or Bl repeat on the opposite strand to that encoding the AFP gene in the first intervening sequence.",

author = "Young, {Peter R.} and Scott, {Richard W.} and Hamer, {Dean H.} and Tilghman, {Shirley M.}",

note = "Funding Information: ACKNOWLEDGEMENTS This work was supported by NCI grant CA-28050, a Basil O'Connor Starter Grant, NIH grants CA-06927 and RR-05539 awarded to the Institute for Cancer Research and an appropriation from the Commonwealth of Pennsylvania. S.M.T. is the recipient of an American Cancer Society Junior Faculty Award, P.R.Y. and R.W.S. are U.S.P.H.S. postdoctoral fellows.",

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doi = "10.1093/nar/10.10.3099",

language = "English (US)",

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T1 - Construction and expression in vivo of an internally deleted mouse α-fetoprotein gene

T2 - Presence of a transcribed alu-like repeat within the first intervening sequence

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AU - Scott, Richard W.

AU - Hamer, Dean H.

AU - Tilghman, Shirley M.

N1 - Funding Information: ACKNOWLEDGEMENTS This work was supported by NCI grant CA-28050, a Basil O'Connor Starter Grant, NIH grants CA-06927 and RR-05539 awarded to the Institute for Cancer Research and an appropriation from the Commonwealth of Pennsylvania. S.M.T. is the recipient of an American Cancer Society Junior Faculty Award, P.R.Y. and R.W.S. are U.S.P.H.S. postdoctoral fellows.

PY - 1982/5/25

Y1 - 1982/5/25

N2 - An α-fetoprotein (AFP)* 'minigene' was constructed by joining the first two exons along with 0.9 kilobases of 5′ flanking sequence of the mouse AFP gene to its last exon and 0.4 kilobases of 3′ flanking sequence. This 'minigene' was tested for activity by inserting it into an SV40 vector and infecting African green monkey kidney cells. Correct initiation, termination, polyadenylation and splicing were observed. Furthermore a 220 nucleotide transcript was detected and mapped to a mouse Alu-like or Bl repeat on the opposite strand to that encoding the AFP gene in the first intervening sequence.

AB - An α-fetoprotein (AFP)* 'minigene' was constructed by joining the first two exons along with 0.9 kilobases of 5′ flanking sequence of the mouse AFP gene to its last exon and 0.4 kilobases of 3′ flanking sequence. This 'minigene' was tested for activity by inserting it into an SV40 vector and infecting African green monkey kidney cells. Correct initiation, termination, polyadenylation and splicing were observed. Furthermore a 220 nucleotide transcript was detected and mapped to a mouse Alu-like or Bl repeat on the opposite strand to that encoding the AFP gene in the first intervening sequence.

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Construction and expression in vivo of an internally deleted mouse α-fetoprotein gene: Presence of a transcribed alu-like repeat within the first intervening sequence

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