Construction and expression in vivo of an internally deleted mouse α-fetoprotein gene: Presence of a transcribed alu-like repeat within the first intervening sequence

Peter R. Young, Richard W. Scott, Dean H. Hamer, Shirley M. Tilghman

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

An α-fetoprotein (AFP)* 'minigene' was constructed by joining the first two exons along with 0.9 kilobases of 5′ flanking sequence of the mouse AFP gene to its last exon and 0.4 kilobases of 3′ flanking sequence. This 'minigene' was tested for activity by inserting it into an SV40 vector and infecting African green monkey kidney cells. Correct initiation, termination, polyadenylation and splicing were observed. Furthermore a 220 nucleotide transcript was detected and mapped to a mouse Alu-like or Bl repeat on the opposite strand to that encoding the AFP gene in the first intervening sequence.

Original languageEnglish (US)
Pages (from-to)3099-3116
Number of pages18
JournalNucleic acids research
Volume10
Issue number10
DOIs
StatePublished - May 25 1982

All Science Journal Classification (ASJC) codes

  • Genetics

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