The integrins are a family of αβ heterodimeric transmembrane receptors that link extracellular matrix (ECM) proteins to the cytoskeleton and orchestrate cell behaviors. It's been suggested that integrins interact with Rho family small GTPases, such as Rho and Rac. We took advantage of a C. elegans nematode line expressing HA-βtail, a β integrin transgene inhibiting the functions of endogenous integrins, to determine the combined effects of reducing PAT-3 β integrin and Rac pathway activities. Double mutants of HA-βtail and unc-73, a guanine nucleotide exchange factor GEF for MIG-2/Rac, had body wall and vulval muscle abnormalities. On the other hand, HA-βtail combined with mutant CED-5, another Rac interacting protein, showed ovulation defects and sterility. RNA-mediated interference (RNAi) of pat-3 on Rac mutant backgrounds also affected gonad structure and function. These results show a functional link between integrins and Rac signaling in muscles and gonads. Furthermore, data showing distinct phenotypes of HA-βtail with unc-73 versus ced-5 suggest some tissue-specificity in the usage of Rac signaling pathways.
All Science Journal Classification (ASJC) codes
- Molecular Biology
- Cell migration
- Rac GTPase