Conferral of transposable properties to a chromosomal gene in Escherichia coli

Leonard P. Guarente, Ralph R. Isberg, Michael Syvanen, Thomas J. Silhavy

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

A normally stable gene of Escherichia coli was converted into a transposable element. A bacterial strain was constructed in which the malK gene was flanked on each side by the transposable element Tn5. The resulting Tn5-malK+-Tn5 structure (Tn651) became a transposable element with properties very similar to those of Tn5 itself. Tn651 transposes into regions of both the E. coli chromosome and bacteriophage lambda and is able to induce mutations. Transposition of Tn651 does not require the product of recA. Based on a physical analysis of lambda Tn651 DNA it is shown that the two Tn5s flanking the malK gene are in inverted orientation. In these experiments a new derivative of bacteriophage lambda is used that can accept a 14 kilobase insertion in vivo and still yield a plaque-forming transducing particle.

Original languageEnglish (US)
Pages (from-to)235-248
Number of pages14
JournalJournal of Molecular Biology
Volume141
Issue number3
DOIs
StatePublished - Jan 1 1980
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

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