Computational Models for Neurogenic Gene Expression in the Drosophila Embryo

Robert P. Zinzen, Kate Senger, Mike Levine, Dmitri Papatsenko

Research output: Contribution to journalArticle

128 Scopus citations

Abstract

The early Drosophila embryo is emerging as a premiere model system for the computational analysis of gene regulation in development because most of the genes, and many of the associated regulatory DNAs, that control segmentation and gastrulation are known [1-5]. The comprehensive elucidation of Drosophila gene networks provides an unprecedented opportunity to apply quantitative models to metazoan enhancers that govern complex patterns of gene expression during development [6-11]. Models based on the fractional occupancy of defined DNA binding sites have been used to describe the regulation of the lac operon in E. coli and the lysis/lysogeny switch of phage lambda [12-15]. Here, we apply similar models to enhancers regulated by the Dorsal gradient in the ventral neurogenic ectoderm (vNE) of the early Drosophila embryo. Quantitative models based on the fractional occupancy of Dorsal, Twist, and Snail binding sites raise the possibility that cooperative interactions among these regulatory proteins mediate subtle differences in the vNE expression patterns. Variations in cooperativity may be attributed to differences in the detailed linkage of Dorsal, Twist, and Snail binding sites in vNE enhancers. We propose that binding site occupancy is the key rate-limiting step for establishing localized patterns of gene expression in the early Drosophila embryo.

Original languageEnglish (US)
Pages (from-to)1358-1365
Number of pages8
JournalCurrent Biology
Volume16
Issue number13
DOIs
StatePublished - Jul 11 2006
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Keywords

  • DEVBIO

Fingerprint Dive into the research topics of 'Computational Models for Neurogenic Gene Expression in the Drosophila Embryo'. Together they form a unique fingerprint.

  • Cite this