Complementary proteomic analysis of protein complexes

Todd M. Greco, Yana Miteva, Frank L. Conlon, Ileana M. Cristea

Research output: Chapter in Book/Report/Conference proceedingChapter

19 Scopus citations


Proteomic characterization of protein complexes leverages the versatile platform of liquid chromatography-tandem mass spectrometry to elucidate molecular and cellular signaling processes underlying the dynamic regulation of macromolecular assemblies. Here, we describe a complementary proteomic approach optimized for immunoisolated protein complexes. As the relative complexity, abundance, and physiochemical properties of proteins can vary significantly between samples, we have provided (1) complementary sample preparation workflows, (2) detailed steps for HPLC and mass spectrometric method development, and (3) a bioinformatic workflow that provides confident peptide/protein identification paired with unbiased functional gene ontology analysis. This protocol can also be extended for characterization of larger complexity samples from whole cell or tissue Xenopus proteomes.

Original languageEnglish (US)
Title of host publicationXenopus Protocols
Subtitle of host publicationPost-Genomic Approaches
PublisherHumana Press Inc.
Number of pages17
ISBN (Print)9781617799914
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Genetics
  • Molecular Biology


  • Bioinformatics
  • HPLC
  • In-gel digestion
  • In-solution digestion
  • Mass spectrometry
  • Protein complex
  • Protein interactions
  • Proteomics
  • Xenopus


Dive into the research topics of 'Complementary proteomic analysis of protein complexes'. Together they form a unique fingerprint.

Cite this