Comparison of the interactions of the adenovinis type 2 major core protein and its precursor with DNA

Pradeep K. Chatterjee, U. C. Yang, S. J. Flint

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The interactions of the major core protein of adenovirus type 2 (Ad2) protein VII, and its precursor, protein pre-VII, with viral DHA, were studied using UV light induoed crossllnklng of 32P-labelled ollgonuoleotldes to the proteins. Proteolytio fragments of these two proteins that oontaln DNA-bindlng donalns were Identified by virtue of their oovalently attaohed, alkali-resistant 32P-radioaotivity. The overall effioienoy of orossllnking of protein pre-VII to DHA, in H2ts1 virions assembled at 39°C, was comparable to that of the orosslinklng of protein Til to DHA in Ad2 virions. However, a protease T8 fragment comprising the H-terminal half of protein pre-VII orosslinked to DHA at least ten tines Bore efficiently than the corresponding B-termlnal fragnent of protein Til, whlob is truncated by the reaoval of 23 amino adds fron the H-temlnus of protein pre-VII during virion saturation.

Original languageEnglish (US)
Pages (from-to)2721-2735
Number of pages15
JournalNucleic acids research
Volume14
Issue number6
DOIs
StatePublished - Mar 11 1986

All Science Journal Classification (ASJC) codes

  • Genetics

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