TY - JOUR
T1 - Comparing genomic expression patterns across species identifies shared transcriptional profile in aging
AU - McCarroll, Steven A.
AU - Murphy, Coleen T.
AU - Zou, Sige
AU - Pletcher, Scott D.
AU - Chin, Chen Shan
AU - Jan, Yuh Nung
AU - Kenyon, Cynthia
AU - Bargmann, Cornelia I.
AU - Li, Hao
PY - 2004/2/1
Y1 - 2004/2/1
N2 - We developed a method for systematically comparing gene expression patterns across organisms using genome-wide comparative analysis of DNA microarray experiments. We identified analogous gene expression programs comprising shared patterns of regulation across orthologous genes. Biological features of these patterns could be identified as highly conserved subpatterns that correspond to Gene Ontology categories. Here, we demonstrate these methods by analyzing a specific biological process, aging, and show that similar analysis can be applied to a range of biological processes. We found that two highly diverged animals, the nematode Caenorhabditis elegans and the fruit fly Drosophila melanogaster, implement a shared adult-onset expression program of genes involved in mitochondrial metabolism, DNA repair, catabolism, peptidolysis and cellular transport. Most of these changes were implemented early in adulthood. Using this approach to search databases of gene expression data, we found conserved transcriptional signatures in larval development, embryogenesis, gametogenesis and MRNA degradation.
AB - We developed a method for systematically comparing gene expression patterns across organisms using genome-wide comparative analysis of DNA microarray experiments. We identified analogous gene expression programs comprising shared patterns of regulation across orthologous genes. Biological features of these patterns could be identified as highly conserved subpatterns that correspond to Gene Ontology categories. Here, we demonstrate these methods by analyzing a specific biological process, aging, and show that similar analysis can be applied to a range of biological processes. We found that two highly diverged animals, the nematode Caenorhabditis elegans and the fruit fly Drosophila melanogaster, implement a shared adult-onset expression program of genes involved in mitochondrial metabolism, DNA repair, catabolism, peptidolysis and cellular transport. Most of these changes were implemented early in adulthood. Using this approach to search databases of gene expression data, we found conserved transcriptional signatures in larval development, embryogenesis, gametogenesis and MRNA degradation.
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U2 - 10.1038/ng1291
DO - 10.1038/ng1291
M3 - Article
C2 - 14730301
AN - SCOPUS:0842267618
VL - 36
SP - 197
EP - 204
JO - Nature Genetics
JF - Nature Genetics
SN - 1061-4036
IS - 2
ER -