Community level analysis: Genetic and biogeochemical approaches to investigate community composition and function in aerobic ammonia oxidation

Bettie Ward, Gregory D. O'Mullan

Research output: Contribution to journalReview article

15 Scopus citations

Abstract

Aerobic ammonia oxidation is the process that converts ammonium to nitrate and thus links the regeneration of organic nitrogen to fixed nitrogen loss by denitrification. It is performed by a phylogenetically restricted group of Proteobacteria (ammonia-oxidizing bacteria, AOB) that are autotrophic and obligately aerobic. This chapter describes methods for the measurement of ammonia oxidation in the environment, with a focus on seawater systems and stable isotopic tracer methods. It also summarizes the current state of molecular ecological approaches for detection of AOB in the environment and characterization of the composition of AOB assemblages.

Original languageEnglish (US)
Pages (from-to)395-413
Number of pages19
JournalMethods in enzymology
Volume397
DOIs
StatePublished - Jan 1 2005

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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