ColE1 cloning of a ribosomal RNA promoter region from λrifd18 by selection for lambda integration and excision functions

Gad Glaser, Lynn William Enquist, Michael Cashel

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

The expression of the ribosomal RNA gene carried by the lambda transducing phage λrifd18 is shown to be subject to stringent amino acid control. λrifd18 DNA was digested with endonuclease EcoRI and ligated to similarly restricted ColE1 plasmid DNA. Selection for expression of lambda integration and excision gene activity carried by the same DNA fragment results in cloning of the promoter proximal portion of the 16S ribosomal RNA gene. The resulting chimera expresses λ integration and excision functions as well as encoding the promoter proximal half of a 16S ribosomal RNA gene.

Original languageEnglish (US)
Pages (from-to)159-172
Number of pages14
JournalGene
Volume2
Issue number3-4
DOIs
StatePublished - Jan 1 1977
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Genetics

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