Cog5-cog7 crystal structure reveals interactions essential for the function of a multisubunit tethering complex

Jun Yong Ha, Irina D. Pokrovskaya, Leslie K. Climer, Gregory R. Shimamura, Tetyana Kudlyk, Philip D. Jeffrey, Vladimir V. Lupashin, Frederick M. Hughson

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

The conserved oligomeric Golgi (COG) complex is required, along with SNARE and Sec1/Munc18 (SM) proteins, for vesicle docking and fusion at the Golgi. COG, like other multisubunit tethering complexes (MTCs), is thought to function as a scaffold and/or chaperone to direct the assembly of productive SNARE complexes at the sites of membrane fusion. Reflecting this essential role, mutations in the COG complex can cause congenital disorders of glycosylation. A deeper understanding of COG function and dysfunction will likely depend on elucidating its molecular structure. Despite some progress toward this goal, including EM studies of COG lobe A (subunits 1-4) and higher-resolution structures of portions of Cog2 and Cog4, the structures of COG's eight subunits and the principles governing their assembly are mostly unknown. Here, we report the crystal structure of a complex between two lobe B subunits, Cog5 and Cog7. The structure reveals that Cog5 is a member of the complexes associated with tethering containing helical rods (CATCHR) fold family, with homology to subunits of other MTCs including the Dsl1, exocyst, and Golgi-associated retrograde protein (GARP) complexes. The Cog5-Cog7 interaction is analyzed in relation to the Dsl1 complex, the only other CATCHR-family MTC for which subunit interactions have been characterized in detail. Biochemical and functional studies validate the physiological relevance of the observed Cog5-Cog7 interface, indicate that it is conserved fromyeast to humans, and demonstrate that its disruption in human cells causes defects in trafficking and glycosylation.

Original languageEnglish (US)
Pages (from-to)15762-15767
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number44
DOIs
StatePublished - Nov 4 2014

All Science Journal Classification (ASJC) codes

  • General

Keywords

  • CATCHR
  • COG complex
  • Congenital glycosylation disorder
  • Golgi
  • Vesicle fusion

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