Coding potential of laboratory and clinical strains of human cytomegalovirus

Eain Murphy; Dong Yu; Jane Grimwood; Jeremy Schmutz; Mark Dickson; Michael A. Jarvis; Gabriele Hahn; Jay A. Nelson; Richard M. Myers; Thomas E. Shenk

doi:10.1073/pnas.2136652100

Coding potential of laboratory and clinical strains of human cytomegalovirus

Eain Murphy, Dong Yu, Jane Grimwood, Jeremy Schmutz, Mark Dickson, Michael A. Jarvis, Gabriele Hahn, Jay A. Nelson, Richard M. Myers, Thomas E. Shenk

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425 Scopus citations

Abstract

Six strains of human cytomegalovirus have been sequenced, including two laboratory strains (AD169 and Towne) that have been extensively passaged in fibroblasts and four clinical isolates that have been passaged to a limited extent in the laboratory (Toledo, FIX, PH, and TR). All of the sequenced viral genomes have been cloned as infectious bacterial artificial chromosomes. A total of 252 ORFs with the potential to encode proteins have been identified that are conserved in all four clinical isolates of the virus. Multiple sequence alignments revealed substantial variation in the amino acid sequences encoded by many of the conserved ORFs.

Original language	English (US)
Pages (from-to)	14976-14981
Number of pages	6
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	100
Issue number	25
DOIs	https://doi.org/10.1073/pnas.2136652100
State	Published - Dec 9 2003

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abstract = "Six strains of human cytomegalovirus have been sequenced, including two laboratory strains (AD169 and Towne) that have been extensively passaged in fibroblasts and four clinical isolates that have been passaged to a limited extent in the laboratory (Toledo, FIX, PH, and TR). All of the sequenced viral genomes have been cloned as infectious bacterial artificial chromosomes. A total of 252 ORFs with the potential to encode proteins have been identified that are conserved in all four clinical isolates of the virus. Multiple sequence alignments revealed substantial variation in the amino acid sequences encoded by many of the conserved ORFs.",

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T1 - Coding potential of laboratory and clinical strains of human cytomegalovirus

AU - Murphy, Eain

AU - Yu, Dong

AU - Grimwood, Jane

AU - Schmutz, Jeremy

AU - Dickson, Mark

AU - Jarvis, Michael A.

AU - Hahn, Gabriele

AU - Nelson, Jay A.

AU - Myers, Richard M.

AU - Shenk, Thomas E.

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N2 - Six strains of human cytomegalovirus have been sequenced, including two laboratory strains (AD169 and Towne) that have been extensively passaged in fibroblasts and four clinical isolates that have been passaged to a limited extent in the laboratory (Toledo, FIX, PH, and TR). All of the sequenced viral genomes have been cloned as infectious bacterial artificial chromosomes. A total of 252 ORFs with the potential to encode proteins have been identified that are conserved in all four clinical isolates of the virus. Multiple sequence alignments revealed substantial variation in the amino acid sequences encoded by many of the conserved ORFs.

AB - Six strains of human cytomegalovirus have been sequenced, including two laboratory strains (AD169 and Towne) that have been extensively passaged in fibroblasts and four clinical isolates that have been passaged to a limited extent in the laboratory (Toledo, FIX, PH, and TR). All of the sequenced viral genomes have been cloned as infectious bacterial artificial chromosomes. A total of 252 ORFs with the potential to encode proteins have been identified that are conserved in all four clinical isolates of the virus. Multiple sequence alignments revealed substantial variation in the amino acid sequences encoded by many of the conserved ORFs.

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Coding potential of laboratory and clinical strains of human cytomegalovirus

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