Cocrystals of Escherichia coli trp repressor bound to an alternative operator DNA sequence

Jannette Carey, Nick Combatti, Dale E.A. Lewis, Catherine L. Lawson

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Cocrystals of a 2:1 complex of trp repressor dimers with a DNA duplex containing a single, central operator half-site sequence are described. Crystals with different morphologies grew under diverse crystallization conditions within days to weeks by hanging-drop vapor-diffusion. Twinned rods split along their longitudinal cleft produce single crystals with space group C2 and unit cell dimensions a = 112.34 Å, b = 90.16 Å, c = 58.65 Å and β = 113.92°. The crystals diffract to 2.4 Å and are thus suitable for structural analysis by X-ray diffraction. Several heavy-atom derivative cocrystals have been obtained with iodouridine-substituted DNAs.

Original languageEnglish (US)
Pages (from-to)496-498
Number of pages3
JournalJournal of Molecular Biology
Volume234
Issue number2
DOIs
StatePublished - Nov 20 1993

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Keywords

  • Biased random search
  • Cooperativity
  • DNA sequence context
  • DNA-protein cocrystallization

Fingerprint Dive into the research topics of 'Cocrystals of Escherichia coli trp repressor bound to an alternative operator DNA sequence'. Together they form a unique fingerprint.

Cite this