Co-regulated transcriptional networks contribute to natural genetic variation in Drosophila sleep

Susan T. Harbison, Mary Anna Carbone, Julien F. Ayroles, Eric A. Stone, Richard F. Lyman, Trudy F.C. Mackay

Research output: Contribution to journalArticle

73 Scopus citations

Abstract

Sleep disorders are common in humans, and sleep loss increases the risk of obesity and diabetes. Studies in Drosophila have revealed molecular pathways and neural tissues regulating sleep; however, genes that maintain genetic variation for sleep in natural populations are unknown. Here, we characterized sleep in 40 wild-derived Drosophila lines and observed abundant genetic variation in sleep architecture. We associated sleep with genome-wide variation in gene expression to identify candidate genes. We independently confirmed that molecular polymorphisms in Catsup (Catecholamines up) are associated with variation in sleep and that P-element mutations in four candidate genes affect sleep and gene expression. Transcripts associated with sleep grouped into biologically plausible genetically correlated transcriptional modules. We confirmed co-regulated gene expression using P-element mutants. Quantitative genetic analysis of natural phenotypic variation is an efficient method for revealing candidate genes and pathways.

Original languageEnglish (US)
Pages (from-to)371-375
Number of pages5
JournalNature Genetics
Volume41
Issue number3
DOIs
StatePublished - Mar 2009
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

Fingerprint Dive into the research topics of 'Co-regulated transcriptional networks contribute to natural genetic variation in Drosophila sleep'. Together they form a unique fingerprint.

  • Cite this

    Harbison, S. T., Carbone, M. A., Ayroles, J. F., Stone, E. A., Lyman, R. F., & Mackay, T. F. C. (2009). Co-regulated transcriptional networks contribute to natural genetic variation in Drosophila sleep. Nature Genetics, 41(3), 371-375. https://doi.org/10.1038/ng.330