cis-Acting sites required for osmoregulation of ompF expression in Escherichia coli K-12

K. S. Ostrow, T. J. Silhavy, S. Garrett

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

OmpF and OmpC are major outer membrane proteins which form passive diffusion pores in Escherichia coli K-12. The expression of the structural genes for these proteins, ompF and ompC, is influenced by medium osmotic strength and requires the products of two regulatory genes, ompR and envZ. We have constructed a series of ompF-lacZ fusions containing different regions of ompF to determine sites involved with osmoregulation. These fusions were crossed onto a specialized transducing phage and integrated into the bacterial chromosome in unit copy. By measuring the fluctuations of β-galactosidase activity in lysogens grown in high versus low osmolarity, we have identified three regions which are necessary. Furthermore, we have determined that, although the OmpR activation site is not sufficient, OmpR is probably essential for ompF osmoregulation.

Original languageEnglish (US)
Pages (from-to)1165-1171
Number of pages7
JournalJournal of bacteriology
Volume168
Issue number3
DOIs
StatePublished - 1986

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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