Chromatin structure of the histone genes of D. melanogaster

Babrubahan Samal, Abraham Worcel, Christos Louis, Paul Schedl

Research output: Contribution to journalArticle

100 Scopus citations

Abstract

We have examined the chromatin structure of the histone gene repeat of D. melanogaster using an indirect end-labeling technique. Our results show that each DNA segment of the repeat is packaged into a precisely defined and characteristic structure, as follows: the nontranscribed spacers display a "normal" chromatin arrangement, with each nucleosome precisely positioned on the underlying DNA sequence; the 5′ ends of all five histone genes are in an exposed configuration, highly sensitive to both micrococcal nuclease and DNAase I; and the genes have an "altered" chromatin structure, as indicated by the weak and irregularly spaced nuclease cuts. This well-defined chromatin arrangement is established early in development and is stably maintained throughout the remainder of the D. melanogaster life cycle.

Original languageEnglish (US)
Pages (from-to)401-409
Number of pages9
JournalCell
Volume23
Issue number2
DOIs
StatePublished - Feb 1981

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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