TY - JOUR
T1 - Chromatin structure of the 5S RNA genes of D. melanogaster
AU - Louis, Christos
AU - Schedl, Paul
AU - Samal, Babrubahan
AU - Worcel, Abraham
PY - 1980/1/1
Y1 - 1980/1/1
N2 - The 5S gene cluster of Drosophia melanogaster is a tandem array of a repeat unit made up of a 135 bp gene plus a 238 bp spacer. The length of the 5S repeat (373 bp) equals the average length of a Drosophila dinucleosome. Digestion of Drosophila nuclei with micrococcal nuclease generates discrete 5S RNA gene subfragments when the purified DNA is further cleaved with a single-cut restriction enzyme. We have mapped four micrococcal nuclease-sensitive sites within the 5S repeat: A1, centered at bp −110 (+1 being the G:C bp at the start of the gene); A1′, at bp −80; A2, at bp +80, within the intragenic control region; and B, at −190 bp. These findings suggest that nucleosomes can be positioned on the 5S gene repeat in one of two possible phases, A or B. In the A phase a potential regulatory sequence near the center of the gene is exposed in one of the two linkers of the repeat. In the B phase, in contrast, one of the linkers includes the 5′ end of the gene.
AB - The 5S gene cluster of Drosophia melanogaster is a tandem array of a repeat unit made up of a 135 bp gene plus a 238 bp spacer. The length of the 5S repeat (373 bp) equals the average length of a Drosophila dinucleosome. Digestion of Drosophila nuclei with micrococcal nuclease generates discrete 5S RNA gene subfragments when the purified DNA is further cleaved with a single-cut restriction enzyme. We have mapped four micrococcal nuclease-sensitive sites within the 5S repeat: A1, centered at bp −110 (+1 being the G:C bp at the start of the gene); A1′, at bp −80; A2, at bp +80, within the intragenic control region; and B, at −190 bp. These findings suggest that nucleosomes can be positioned on the 5S gene repeat in one of two possible phases, A or B. In the A phase a potential regulatory sequence near the center of the gene is exposed in one of the two linkers of the repeat. In the B phase, in contrast, one of the linkers includes the 5′ end of the gene.
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U2 - 10.1016/0092-8674(80)90349-9
DO - 10.1016/0092-8674(80)90349-9
M3 - Article
C2 - 6778618
AN - SCOPUS:0019174807
VL - 22
SP - 387
EP - 392
JO - Cell
JF - Cell
SN - 0092-8674
IS - 2
ER -