TY - JOUR
T1 - Chemical Approaches To Investigate Post-transcriptional RNA Regulation
AU - Kleiner, Ralph E.
N1 - Funding Information:
The Kleiner lab graciously acknowledges the support of the NIH (R01 GM132189), NSF (MCB-1942565), Alfred P. Sloan Foundation, Sidney Kimmel Foundation, Damon Runyon Cancer Research Foundation, Princeton University startup funds, Princeton Catalysis Initiative, and Princeton University Innovation Fund for New Ideas in the Natural Sciences. We thank all current and former members of the Kleiner lab for their contributions to the work discussed herein.
Publisher Copyright:
© 2023 American Chemical Society.
PY - 2023/8/18
Y1 - 2023/8/18
N2 - RNA plays a central role in biological processes, and its activity is regulated by a host of diverse chemical and biochemical mechanisms including post-transcriptional modification and interactions with RNA-binding proteins. Here, we describe our efforts to illuminate RNA biology through the application of chemical tools, focusing on post-transcriptional regulatory mechanisms. We describe the development of an activity-based protein profiling approach for discovery and characterization of RNA-modifying enzymes. Next, we highlight novel approaches for RNA imaging based upon metabolic labeling with modified nucleosides and engineering of the nucleotide salvage pathway. Finally, we discuss profiling RNA-protein interactions using small molecule-dependent RNA editing and synthetic photo-cross-linkable oligonucleotide probes. Our work provides enabling technologies for deciphering the complexity of RNA and its diverse functions in biology.
AB - RNA plays a central role in biological processes, and its activity is regulated by a host of diverse chemical and biochemical mechanisms including post-transcriptional modification and interactions with RNA-binding proteins. Here, we describe our efforts to illuminate RNA biology through the application of chemical tools, focusing on post-transcriptional regulatory mechanisms. We describe the development of an activity-based protein profiling approach for discovery and characterization of RNA-modifying enzymes. Next, we highlight novel approaches for RNA imaging based upon metabolic labeling with modified nucleosides and engineering of the nucleotide salvage pathway. Finally, we discuss profiling RNA-protein interactions using small molecule-dependent RNA editing and synthetic photo-cross-linkable oligonucleotide probes. Our work provides enabling technologies for deciphering the complexity of RNA and its diverse functions in biology.
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U2 - 10.1021/acschembio.3c00406
DO - 10.1021/acschembio.3c00406
M3 - Review article
C2 - 37540831
AN - SCOPUS:85168255626
SN - 1554-8929
VL - 18
SP - 1684
EP - 1697
JO - ACS chemical biology
JF - ACS chemical biology
IS - 8
ER -