Chemical Approaches To Investigate Post-transcriptional RNA Regulation

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RNA plays a central role in biological processes, and its activity is regulated by a host of diverse chemical and biochemical mechanisms including post-transcriptional modification and interactions with RNA-binding proteins. Here, we describe our efforts to illuminate RNA biology through the application of chemical tools, focusing on post-transcriptional regulatory mechanisms. We describe the development of an activity-based protein profiling approach for discovery and characterization of RNA-modifying enzymes. Next, we highlight novel approaches for RNA imaging based upon metabolic labeling with modified nucleosides and engineering of the nucleotide salvage pathway. Finally, we discuss profiling RNA-protein interactions using small molecule-dependent RNA editing and synthetic photo-cross-linkable oligonucleotide probes. Our work provides enabling technologies for deciphering the complexity of RNA and its diverse functions in biology.

Original languageEnglish (US)
Pages (from-to)1684-1697
Number of pages14
JournalACS chemical biology
Issue number8
StatePublished - Aug 18 2023

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Biochemistry


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