TY - JOUR
T1 - Chemical Approaches To Investigate Post-transcriptional RNA Regulation
AU - Kleiner, Ralph E.
N1 - Publisher Copyright:
© 2023 American Chemical Society.
PY - 2023/8/18
Y1 - 2023/8/18
N2 - RNA plays a central role in biological processes, and its activity is regulated by a host of diverse chemical and biochemical mechanisms including post-transcriptional modification and interactions with RNA-binding proteins. Here, we describe our efforts to illuminate RNA biology through the application of chemical tools, focusing on post-transcriptional regulatory mechanisms. We describe the development of an activity-based protein profiling approach for discovery and characterization of RNA-modifying enzymes. Next, we highlight novel approaches for RNA imaging based upon metabolic labeling with modified nucleosides and engineering of the nucleotide salvage pathway. Finally, we discuss profiling RNA-protein interactions using small molecule-dependent RNA editing and synthetic photo-cross-linkable oligonucleotide probes. Our work provides enabling technologies for deciphering the complexity of RNA and its diverse functions in biology.
AB - RNA plays a central role in biological processes, and its activity is regulated by a host of diverse chemical and biochemical mechanisms including post-transcriptional modification and interactions with RNA-binding proteins. Here, we describe our efforts to illuminate RNA biology through the application of chemical tools, focusing on post-transcriptional regulatory mechanisms. We describe the development of an activity-based protein profiling approach for discovery and characterization of RNA-modifying enzymes. Next, we highlight novel approaches for RNA imaging based upon metabolic labeling with modified nucleosides and engineering of the nucleotide salvage pathway. Finally, we discuss profiling RNA-protein interactions using small molecule-dependent RNA editing and synthetic photo-cross-linkable oligonucleotide probes. Our work provides enabling technologies for deciphering the complexity of RNA and its diverse functions in biology.
UR - http://www.scopus.com/inward/record.url?scp=85168255626&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85168255626&partnerID=8YFLogxK
U2 - 10.1021/acschembio.3c00406
DO - 10.1021/acschembio.3c00406
M3 - Review article
C2 - 37540831
AN - SCOPUS:85168255626
SN - 1554-8929
VL - 18
SP - 1684
EP - 1697
JO - ACS chemical biology
JF - ACS chemical biology
IS - 8
ER -