Characterization of the intergenic RNA profile at abdominal-A and abdominal-B in the Drosophila bithorax complex

Esther Bae, Vincent C. Calhoun, Michael Levine, Edward B. Lewis, Robert A. Drewell

Research output: Contribution to journalArticle

111 Scopus citations

Abstract

The correct spatial expression of two Drosophila bithorax complex (BX-C) genes, abdominal-A (abdA) and Abdominal-B (AbdB), is dependent on the 100-kb intergenic infraabdominal (iab) region. The iab region is known to contain a number of different domains (iab2 through iab8) that harbor cis-regulatory elements responsible for directing expression of abdA and AbdB in the second through eighth abdominal segments. Here, we use in situ hybridization to perform high-resolution mapping of the transcriptional activity in the iab control regions. We show that transcription of the control regions themselves is abundant and precedes activation of the abdA and AbdB genes. As with the homeotic genes of the BX-C, the transcription patterns of the RNAs from the iab control regions demonstrate colinearity with the sequence of the iab regions along the chromosome and the domains in the embryo under the control of the specific iab regions. These observations suggest that the intergenic RNAs may play a role in initiating cis regulation at the BX-C early in development.

Original languageEnglish (US)
Pages (from-to)16847-16852
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume99
Issue number26
DOIs
StatePublished - Dec 24 2002

All Science Journal Classification (ASJC) codes

  • General

Fingerprint Dive into the research topics of 'Characterization of the intergenic RNA profile at abdominal-A and abdominal-B in the Drosophila bithorax complex'. Together they form a unique fingerprint.

  • Cite this