Characterization of the helicase activity and anti-telomerase properties of Yeast Pif1p in Vitro

Jean Baptiste Boulé, Virginia A. Zakian

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations

Abstract

Pif1p is the prototype member of a family of helicases that is highly conserved from yeast to humans. In yeast, Pif1p is involved in many aspects of the preservation of genome stability. In particular, Pif1p is involved in the maintenance of mitochondrial DNA and in the direct inhibition of telomerase at telomeres and double-stranded breaks. Here we describe methods to purify Pif1p and study in vitro its enzymatic properties and functional interaction with telomerase.

Original languageEnglish (US)
Title of host publicationHelicases
Subtitle of host publicationMethods and Protocols
Pages359-376
Number of pages18
DOIs
StatePublished - 2010

Publication series

NameMethods in Molecular Biology
Volume587
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • Pif1p
  • Yeast
  • helicase
  • oligonucleotide substrate-based radiometric assays
  • telomerase

Fingerprint Dive into the research topics of 'Characterization of the helicase activity and anti-telomerase properties of Yeast Pif1p in Vitro'. Together they form a unique fingerprint.

Cite this