Characterization of the cellulolytic and hydrogen-producing activities of six mesophilic Clostridium species

Zhiyong Ren, T. E. Ward, B. E. Logan, J. M. Regan

Research output: Contribution to journalArticlepeer-review

69 Scopus citations

Abstract

Aims: To characterize cellulolytic, hydrogen-producing clostridia on a comparable basis. Methods and Results: H2 production from cellulose by six mesophilic clostridia was characterized in standardized batch experiments using MN301 cellulose, Avicel and cellobiose. Daily H2 production, substrate degradation, biomass production and the end-point distribution of soluble fermentation products varied with species and substrates. All species produced a significant amount of H2 from cellobiose, with Clostridium acetobutylicum achieving the highest H2 yield of 2.3 mol H 2 mol-1 hexose, but it did not degrade cellulose. Clostridium cellulolyticum and Clostridium populeti catalysed the highest H 2 production from cellulose, with yields of 1.7 and 1.6 mol H 2mol-1 hexose from MN301 and 1.6 and 1.4 mol H2 mol-1 hexose from Avicel, respectively. These species also achieved 25-100% higher H2 production rates from cellulose than the other species. Conclusions: These cellulolytic, hydrogen-producing clostridia varied in H2 production, with Cl. cellulolyticum and Cl. populeti achieving the highest H2 yields and cellulose degradation. Significance and Impact of the Study: The fermentation of cellulosic materials presents a means of H2 production from renewable resources. This standardized comparison provides a quantitative baseline for improving H2 production from cellulose through medium and process optimization and metabolic engineering.

Original languageEnglish (US)
Pages (from-to)2258-2266
Number of pages9
JournalJournal of Applied Microbiology
Volume103
Issue number6
DOIs
StatePublished - Dec 2007

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Applied Microbiology and Biotechnology

Keywords

  • Biomass
  • Cellulose
  • Clostridia
  • Fermentation
  • Hydrogen

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