Characterization of single stranded viral DNA sequences present during replication of adenovirus types 2 and 5

Jane Flint, S. M. Berget, P. A. Sharp

Research output: Contribution to journalArticle

Abstract

Replication intermediates of adenovirus DNA apparently contain extensive stretches of single stranded DNA. Such single stranded viral DNA sequences homologous to different regions of the viral genome present in adenovirus infected cells during viral DNA replication have therefore been characterized by hybridization to the separated strands of restriction endonuclease fragments of 32P labeled adenovirus types 2 and 5 DNA. Saturation hybridization experiments with infected cell DNA extracted at late times suggest that all regions of the adenovirus genome are represented in the single stranded fraction, but at unequal frequencies. This nonuniform representation has been characterized in more detail with self annealed, total cell DNA extracted 18 hr after adenovirus type 2 infection: the concentration of single stranded sequences homologous to different regions of the viral genome was determined by comparing the rates of hybridization of 32P labeled, single stranded DNA probes with such self annealed 18 hr DNA to the rates of hybridization of the same probes with equal concentrations of their complements.

Original languageEnglish (US)
Pages (from-to)559-571
Number of pages13
JournalCell
Volume9
Issue number4 (I)
StatePublished - Jan 1 1976

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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