It is now feasible, through use of recombinant DNA technology, to perform direct genomic comparisons between MSV isolates. We have cloned the integrated proviral DNA sequences of two MSV isolates, m1 and HT1 in bacteriophage λ. Both cloned fragments isolated from transformed mink cells possess full copies of the sarcoma viruses along with flanking host sequences. These cloned fragments have provided the means for direct comparison of the MSV isolates, information on the integration of MSV, and a method of identifying the regions of the viral genome possessing full transforming potential in tissue culture.
|Original language||English (US)|
|Number of pages||11|
|Journal||Symposia on Quantitative Biology|
|State||Published - Dec 1 1979|
All Science Journal Classification (ASJC) codes
- Molecular Biology