Characterization of integrated Moloney sarcoma proviruses and flanking host sequences cloned in bacteriophage λ

G. F. Vande Woude, M. Oskarsson, W. L. McClements, L. W. Enquist, D. G. Blair, P. J. Fischinger, J. V. Maizel, M. Sullivan

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

It is now feasible, through use of recombinant DNA technology, to perform direct genomic comparisons between MSV isolates. We have cloned the integrated proviral DNA sequences of two MSV isolates, m1 and HT1 in bacteriophage λ. Both cloned fragments isolated from transformed mink cells possess full copies of the sarcoma viruses along with flanking host sequences. These cloned fragments have provided the means for direct comparison of the MSV isolates, information on the integration of MSV, and a method of identifying the regions of the viral genome possessing full transforming potential in tissue culture.

Original languageEnglish (US)
Pages (from-to)735-745
Number of pages11
JournalSymposia on Quantitative Biology
Volume44
Issue number2
StatePublished - Dec 1 1979

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics

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    Vande Woude, G. F., Oskarsson, M., McClements, W. L., Enquist, L. W., Blair, D. G., Fischinger, P. J., Maizel, J. V., & Sullivan, M. (1979). Characterization of integrated Moloney sarcoma proviruses and flanking host sequences cloned in bacteriophage λ. Symposia on Quantitative Biology, 44(2), 735-745.