Characterization of coliphage lambda hybrids carrying DNA fragments from Herpes simplex virus type 1 defective interfering particles

K. J. Denniston, M. J. Madden, L. W. Enquist, G. Vande Woude

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

We describe the characterization of 34 hybrid λ bacteriophages carrying EcoRI fragments obtained from DNA of defective interfering particles of the Patton strain of Herpes simplex virus type 1 (HSV-1). All cloned fragments contained S region terminal repeat sequences (TRs) fused to unique HSV-1 DNA. Several fragments contained deletions and rearrangements not described previously for DNA of HSV-1 defective interfering particles. A model describing the generation of defective interfering DNA based on recombination events involving the terminal "a" sequence is presented.

Original languageEnglish (US)
Pages (from-to)365-378
Number of pages14
JournalGene
Volume15
Issue number4
DOIs
StatePublished - Dec 1981
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Genetics

Keywords

  • "a" sequences
  • S region DNA, restriction enzyme mapping
  • defective DNA
  • λgtWES hybrids

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