Characterization of coliphage lambda hybrids carrying DNA fragments from Herpes simplex virus type 1 defective interfering particles

K. J. Denniston; M. J. Madden; L. W. Enquist; G. Vande Woude

doi:10.1016/0378-1119(81)90180-3

Characterization of coliphage lambda hybrids carrying DNA fragments from Herpes simplex virus type 1 defective interfering particles

K. J. Denniston, M. J. Madden, L. W. Enquist, G. Vande Woude

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

We describe the characterization of 34 hybrid λ bacteriophages carrying EcoRI fragments obtained from DNA of defective interfering particles of the Patton strain of Herpes simplex virus type 1 (HSV-1). All cloned fragments contained S region terminal repeat sequences (TR_s) fused to unique HSV-1 DNA. Several fragments contained deletions and rearrangements not described previously for DNA of HSV-1 defective interfering particles. A model describing the generation of defective interfering DNA based on recombination events involving the terminal "a" sequence is presented.

Original language	English (US)
Pages (from-to)	365-378
Number of pages	14
Journal	Gene
Volume	15
Issue number	4
DOIs	https://doi.org/10.1016/0378-1119(81)90180-3
State	Published - Dec 1981

All Science Journal Classification (ASJC) codes

Genetics

Keywords

"a" sequences
S region DNA, restriction enzyme mapping
defective DNA
λgtWES hybrids

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10.1016/0378-1119(81)90180-3

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title = "Characterization of coliphage lambda hybrids carrying DNA fragments from Herpes simplex virus type 1 defective interfering particles",

abstract = "We describe the characterization of 34 hybrid λ bacteriophages carrying EcoRI fragments obtained from DNA of defective interfering particles of the Patton strain of Herpes simplex virus type 1 (HSV-1). All cloned fragments contained S region terminal repeat sequences (TRs) fused to unique HSV-1 DNA. Several fragments contained deletions and rearrangements not described previously for DNA of HSV-1 defective interfering particles. A model describing the generation of defective interfering DNA based on recombination events involving the terminal {"}a{"} sequence is presented.",

keywords = "{"}a{"} sequences, S region DNA, restriction enzyme mapping, defective DNA, λgtWES hybrids",

author = "Denniston, {K. J.} and Madden, {M. J.} and Enquist, {L. W.} and {Vande Woude}, G.",

year = "1981",

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T1 - Characterization of coliphage lambda hybrids carrying DNA fragments from Herpes simplex virus type 1 defective interfering particles

AU - Denniston, K. J.

AU - Madden, M. J.

AU - Enquist, L. W.

AU - Vande Woude, G.

PY - 1981/12

Y1 - 1981/12

N2 - We describe the characterization of 34 hybrid λ bacteriophages carrying EcoRI fragments obtained from DNA of defective interfering particles of the Patton strain of Herpes simplex virus type 1 (HSV-1). All cloned fragments contained S region terminal repeat sequences (TRs) fused to unique HSV-1 DNA. Several fragments contained deletions and rearrangements not described previously for DNA of HSV-1 defective interfering particles. A model describing the generation of defective interfering DNA based on recombination events involving the terminal "a" sequence is presented.

AB - We describe the characterization of 34 hybrid λ bacteriophages carrying EcoRI fragments obtained from DNA of defective interfering particles of the Patton strain of Herpes simplex virus type 1 (HSV-1). All cloned fragments contained S region terminal repeat sequences (TRs) fused to unique HSV-1 DNA. Several fragments contained deletions and rearrangements not described previously for DNA of HSV-1 defective interfering particles. A model describing the generation of defective interfering DNA based on recombination events involving the terminal "a" sequence is presented.

KW - "a" sequences

KW - S region DNA, restriction enzyme mapping

KW - defective DNA

KW - λgtWES hybrids

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JO - Gene

JF - Gene

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ER -

Characterization of coliphage lambda hybrids carrying DNA fragments from Herpes simplex virus type 1 defective interfering particles

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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