Characterization of a pseudorabies virus glycoprotein gene with homology to herpes simplex virus type 1 and type 2 glycoprotein C

A. K. Robbins, R. J. Watson, M. E. Whealy, W. W. Hays, L. W. Enquist

Research output: Contribution to journalArticle

74 Scopus citations

Abstract

A pseudorabies virus (Becker strain) glycoprotein gene was located in the U(L) region at map position 0.40. The gene was identified by using open reading frame Escherichia coli plasmid expression vectors and specific antibody reagents. A 1.55-kilobase unspliced transcript from the gene was detected in pseudorabies virus-infected tissue culture cells. The DNA sequence revealed a single open reading frame of 1,437 base pairs encoding 479 amino acids. The predicted primary translation product has a molecular weight of 50,860 and contains features of a typical herpesvirus glycoprotein. An E. coli expression plasmid was constructed that contained essentially all of the open reading frame for this gene. Antibodies raised in rabbits against the protein expressed in bacteria by this plasmid immunoprecipitated pseudorabies virus-specific glycoproteins of 92,000 and 74,000 daltons from infected cell extracts. It is likely that these two forms represent different glycosylation states of the protein.

Original languageEnglish (US)
Pages (from-to)339-347
Number of pages9
JournalJournal of Virology
Volume58
Issue number2
StatePublished - Nov 19 1986

All Science Journal Classification (ASJC) codes

  • Immunology

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