Characterization of a human cytomegalovirus with phosphorylation site mutations in the immediate-early 2 protein

J. A. Heider, Y. Yu, T. Shenk, J. C. Alwine

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

A human cytomegalovirus mutant (TNsubIE2P) was constructed with alanine substitutions of four residues (T27, S144, T233, and S234) previously shown to be phosphorylated in the immediate-early 2 (IE2) protein. This mutant grew as well as the wild type at both low and high multiplicities of infection. The mutant activated the major immediate-early, UL4, and UL44 promoters to similar levels, and with similar kinetics, as wild-type virus. However, the TNsubIE2P mutant virus transactivated an endogenous simian virus 40 early promoter 4 h earlier and to higher levels than the wild-type virus in infected human fibroblasts. The modification of the IE2 protein by SUMO-1 (i.e., its sumoylated state) was also examined.

Original languageEnglish (US)
Pages (from-to)928-932
Number of pages5
JournalJournal of virology
Volume76
Issue number2
DOIs
StatePublished - 2002
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

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