Characterization and biological activity of cloned simian virus 40 DNA fragments

N. Galanti, G. J. Jonak, K. J. Soprano, J. Floros, L. Kaczmarek, S. Weissman, V. B. Reddy, S. M. Tilghman, R. Baserga

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

The biological activity of fragments of the SV40 genome was determined by manual microinjection of the fragments into the nuclei of mammalian cells. Fragments of the SV40 A gene (that codes for the T antigens) were obtained either directly by digestion with restriction endonucleases or after cloning into plasmid pBR322. Three different biological activities were studied: expression of T antigen, induction of cell DNA synthesis, and, in a few cases, reactivation of repressed ribosomal RNA genes. By using a number of fragments with deletions in the various portions of the SV40 A gene, we have been able to conclude that: 1) the sequences from 0.65 to 0.51 map units are not needed for the induction of cell DNA synthesis; 2) the sequences from 0.42 to 0.17 map units are not needed for the induction of cell DNA synthesis; and 3) the critical sequences for the induction of cell DNA synthesis, 0.51 to 0.42 map units, are different from those necessary for the reactivation of repressed ribosomal RNA genes (0.39-0.33 map units). These results indicate that the information for these two fundamental processes of cell proliferation resides in two separate and distinct domains of the SV40 A gene.

Original languageEnglish (US)
Pages (from-to)6469-6474
Number of pages6
JournalJournal of Biological Chemistry
Volume256
Issue number12
StatePublished - 1981

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Galanti, N., Jonak, G. J., Soprano, K. J., Floros, J., Kaczmarek, L., Weissman, S., Reddy, V. B., Tilghman, S. M., & Baserga, R. (1981). Characterization and biological activity of cloned simian virus 40 DNA fragments. Journal of Biological Chemistry, 256(12), 6469-6474.