Chapter 23 Cyanobactin Ribosomally Synthesized Peptides-A Case of Deep Metagenome Mining

Eric W. Schmidt, Mohamed S. Abou Donia

Research output: Chapter in Book/Report/Conference proceedingChapter

35 Scopus citations

Abstract

Deep metagenome mining is a new method for engineering natural product pathways, focusing on examining symbiotic organisms. The method has been applied to a family of compounds known as cyanobactins, which are ribosomally synthesized peptides produced by cyanobacteria. Often, these cyanobacteria live symbiotically with marine animals, leading to production of natural products in whole animal samples. Here, we focus on methods to identify, clone, and study cyanobactin natural product genes from axenic organisms and metagenomic environments. The application to deep metagenome mining is described, along with other potential targets of this methodology.

Original languageEnglish (US)
Title of host publicationComplex Enzymes in Microbial Natural Product Biosynthesis, Part A
Subtitle of host publicationOverview Articles and Peptides
EditorsDavid Hopwood
Pages575-596
Number of pages22
EditionA
DOIs
StatePublished - 2009

Publication series

NameMethods in Enzymology
NumberA
Volume458
ISSN (Print)0076-6879

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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    Schmidt, E. W., & Abou Donia, M. S. (2009). Chapter 23 Cyanobactin Ribosomally Synthesized Peptides-A Case of Deep Metagenome Mining. In D. Hopwood (Ed.), Complex Enzymes in Microbial Natural Product Biosynthesis, Part A: Overview Articles and Peptides (A ed., pp. 575-596). (Methods in Enzymology; Vol. 458, No. A). https://doi.org/10.1016/S0076-6879(09)04823-X