TY - CHAP
T1 - Chapter 23 Cyanobactin Ribosomally Synthesized Peptides-A Case of Deep Metagenome Mining
AU - Schmidt, Eric W.
AU - Abou Donia, Mohamed S.
N1 - Funding Information:
This work was funded by NIH GM071425. We are grateful for the participation of our colleagues led by Margo Haygood (OHSU) and Jacques Ravel (U. Maryland) in these endeavors.
PY - 2009
Y1 - 2009
N2 - Deep metagenome mining is a new method for engineering natural product pathways, focusing on examining symbiotic organisms. The method has been applied to a family of compounds known as cyanobactins, which are ribosomally synthesized peptides produced by cyanobacteria. Often, these cyanobacteria live symbiotically with marine animals, leading to production of natural products in whole animal samples. Here, we focus on methods to identify, clone, and study cyanobactin natural product genes from axenic organisms and metagenomic environments. The application to deep metagenome mining is described, along with other potential targets of this methodology.
AB - Deep metagenome mining is a new method for engineering natural product pathways, focusing on examining symbiotic organisms. The method has been applied to a family of compounds known as cyanobactins, which are ribosomally synthesized peptides produced by cyanobacteria. Often, these cyanobacteria live symbiotically with marine animals, leading to production of natural products in whole animal samples. Here, we focus on methods to identify, clone, and study cyanobactin natural product genes from axenic organisms and metagenomic environments. The application to deep metagenome mining is described, along with other potential targets of this methodology.
UR - http://www.scopus.com/inward/record.url?scp=64249133292&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=64249133292&partnerID=8YFLogxK
U2 - 10.1016/S0076-6879(09)04823-X
DO - 10.1016/S0076-6879(09)04823-X
M3 - Chapter
C2 - 19374999
AN - SCOPUS:64249133292
SN - 9780123745880
T3 - Methods in Enzymology
SP - 575
EP - 596
BT - Complex Enzymes in Microbial Natural Product Biosynthesis, Part A
A2 - Hopwood, David
ER -