@article{a8fc2aa87bd34af999894377f32c1bdd,
title = "Cellular bases of olfactory circuit assembly revealed by systematic time-lapse imaging",
abstract = "Neural circuit assembly features simultaneous targeting of numerous neuronal processes from constituent neuron types, yet the dynamics is poorly understood. Here, we use the Drosophila olfactory circuit to investigate dynamic cellular processes by which olfactory receptor neurons (ORNs) target axons precisely to specific glomeruli in the ipsi- and contralateral antennal lobes. Time-lapse imaging of individual axons from 30 ORN types revealed a rich diversity in extension speed, innervation timing, and ipsilateral branch locations and identified that ipsilateral targeting occurs via stabilization of transient interstitial branches. Fast imaging using adaptive optics-corrected lattice light-sheet microscopy showed that upon approaching target, many ORN types exhibiting “exploring branches” consisted of parallel microtubule-based terminal branches emanating from an F-actin-rich hub. Antennal nerve ablations uncovered essential roles for bilateral axons in contralateral target selection and for ORN axons to facilitate dendritic refinement of postsynaptic partner neurons. Altogether, these observations provide cellular bases for wiring specificity establishment.",
keywords = "time-lapse imaging, circuit assembly, olfactory system, olfactory receptor neurons, antennal lobe, Drosophilia, growth cone, cytoskeleton, bilateral symmetry, target selection",
author = "Tongchao Li and Fu, {Tian Ming} and Wong, {Kenneth Kin Lam} and Hongjie Li and Qijing Xie and Luginbuhl, {David J.} and Wagner, {Mark J.} and Eric Betzig and Liqun Luo",
note = "Funding Information: We thank N. {\"O}zel and R. Hiesinger for their advice on the explant culture; T. Lecuit, L. Lavis, M. Freeman, Y. Aso, and Bloomington Drosophila Stock Center for reagents; Y. Ge for assistance on fly work; J. Baruni, T. Brown, P. Dong, D. Friedmann, L. Lavis, J. Li, Z. Li, C. McLaughlin, D. Milkie, D. Pederick, K. Shen, Y. Takeo, G. Upadhyayula, R. Zhong for discussions; and T. Lee for sharing equipment at Janelia Research Campus. T.L. and L.L. thank the Yingwei Cui and Hui Zhao Neuroscience Fund for support. H.L. was a Stanford Neuroscience Institute interdisciplinary postdoctoral scholar. E.B. and L.L. are HHMI investigators. This work was supported by NIH ( 1K99DC01883001 to T.L. and R01-DC005982 to L.L.). Funding Information: We thank N. {\"O}zel and R. Hiesinger for their advice on the explant culture; T. Lecuit, L. Lavis, M. Freeman, Y. Aso, and Bloomington Drosophila Stock Center for reagents; Y. Ge for assistance on fly work; J. Baruni, T. Brown, P. Dong, D. Friedmann, L. Lavis, J. Li, Z. Li, C. McLaughlin, D. Milkie, D. Pederick, K. Shen, Y. Takeo, G. Upadhyayula, R. Zhong for discussions; and T. Lee for sharing equipment at Janelia Research Campus. T.L. and L.L. thank the Yingwei Cui and Hui Zhao Neuroscience Fund for support. H.L. was a Stanford Neuroscience Institute interdisciplinary postdoctoral scholar. E.B. and L.L. are HHMI investigators. This work was supported by NIH (1K99DC01883001 to T.L. and R01-DC005982 to L.L.). T.L. and L.L. designed experiments. T.-M.F. and T.L. performed AO-LLSM imaging under the guidance of E.B. K.K.L.W. characterized overlapping of PN dendrites from neighboring glomeruli. H.L. screened PN split GAL4 lines. Q.X. assisted with the fly experiments. D.J.L. helped generating transgenic flies. M.J.W. advised two-photon imaging and statistical analyses. T.L. performed all other experiments and analyzed the data. T.L. and L.L. wrote the paper, with input from all coauthors. L.L. supervised the project. The authors declare no competing interests. We worked to ensure sex balance in the selection of non-human subjects. While citing references scientifically relevant for this work, we also actively worked to promote gender balance in our reference list. Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",
year = "2021",
month = sep,
day = "30",
doi = "10.1016/j.cell.2021.08.030",
language = "English (US)",
volume = "184",
pages = "5107--5121.e14",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "20",
}