Cell-type-specific fibronectin subunits generated by alternative splicing

J. I. Paul, J. E. Schwarzbauer, J. W. Tamkun, R. O. Hynes

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121 Scopus citations


Multiple fibronectin mRNAs arise by alternative splicing of the primary transcript of a single gene. We describe analyses of the contribution of this alternative splicing to fibronectin subunit heterogeneity in three different cell types using antisera directed against specific segments of fibronectin. β-galactosidase-fibronectin fusion proteins produced with the λgtll bacterial expression vector were used as immunogens. One region of alternative splicing accounts for differences in subunit size, while a second contributes to differences between the fibronectins present in blood plasma and in fibronectin cells. We also show, however, that these two regions of alternative splicing do not account for all detectable subunits. We have also used these segment-specific antisera to show that blood platelets contain a spectrum of fibronectin subunits distinct from that found in blood plasma.

Original languageEnglish (US)
Pages (from-to)12258-12265
Number of pages8
JournalJournal of Biological Chemistry
Issue number26
StatePublished - 1986
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology


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