Abstract

The fluid-mosaic model posits a liquid-like plasma membrane, which can flow in response to tension gradients. It is widely assumed that membrane flow transmits local changes in membrane tension across the cell in milliseconds, mediating long-range signaling. Here, we show that propagation of membrane tension occurs quickly in cell-attached blebs but is largely suppressed in intact cells. The failure of tension to propagate in cells is explained by a fluid dynamical model that incorporates the flow resistance from cytoskeleton-bound transmembrane proteins. Perturbations to tension propagate diffusively, with a diffusion coefficient D σ ∼0.024 μm 2 /s in HeLa cells. In primary endothelial cells, local increases in membrane tension lead only to local activation of mechanosensitive ion channels and to local vesicle fusion. Thus, membrane tension is not a mediator of long-range intracellular signaling, but local variations in tension mediate distinct processes in sub-cellular domains.

Original languageEnglish (US)
Pages (from-to)1769-1779.e13
JournalCell
Volume175
Issue number7
DOIs
StatePublished - Dec 13 2018

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Keywords

  • cell mechanics
  • membrane signaling
  • membrane tension
  • porous media
  • rheology

Fingerprint Dive into the research topics of 'Cell Membranes Resist Flow'. Together they form a unique fingerprint.

  • Cite this

    Shi, Z., Graber, Z. T., Baumgart, T., Stone, H. A., & Cohen, A. E. (2018). Cell Membranes Resist Flow. Cell, 175(7), 1769-1779.e13. https://doi.org/10.1016/j.cell.2018.09.054