@inbook{aafd0afcb1cf4a08b9324340f17a9511,
title = "Cell-derived decellularized extracellular matrices",
abstract = "The ability to create cell-derived decellularized matrices in a dish gives researchers the opportunity to possess a bioactive, biocompatible material made up of fibrillar proteins and other factors that recapitulates key features of the native structure and composition of in vivo microenvironments. By using cells in a culture system to provide a natural ECM, decellularization allows for a high degree of customization through the introduction of selected proteins and soluble factors. The culture system, culture medium, cell types, and physical environments can be varied to provide specialized ECMs for wide-ranging applications to study cell–ECM signaling, cell migration, cell differentiation, and tissue engineering purposes. This chapter describes a procedure for performing a detergent and high pH-based extraction that leaves the native, cell-assembled ECM intact while removing cellular materials. We address common evaluation methods for assessing the ECM and its composition as well as potential uses for a decellularized ECM.",
keywords = "Cell–ECM interactions, Decellularization, Extracellular matrix, Scaffold, Tissue engineering",
author = "Harris, {Greg M.} and Irene Raitman and Schwarzbauer, {Jean E.}",
note = "Funding Information: The authors are grateful to Dr. Gary Laevsky, director of the Molecular Biology Confocal Microscopy Core Facility, a Nikon Center of Excellence, for scientific and technical assistance. We also thank the National Institutes of Health (NIH) (R01 CA160611 and P41 EB001046), the New Jersey Commission on Spinal Cord Research (CSCR15IRG002), and the Princeton Dean for Research (Innovation award). I.R. was supported by a Princeton Dept. of Molecular Biology NIH predoctoral training grant (T32 GM007388). G.M.H. was supported by a NJ Center for Biomaterials NIH postdoctoral training grant (T32EB005583). Publisher Copyright: {\textcopyright} 2018 Elsevier Inc.",
year = "2018",
doi = "10.1016/bs.mcb.2017.08.007",
language = "English (US)",
series = "Methods in Cell Biology",
publisher = "Academic Press Inc.",
pages = "97--114",
booktitle = "Methods in Cell Biology",
address = "United States",
}