Cell-derived decellularized extracellular matrices

Greg M. Harris, Irene Raitman, Jean E. Schwarzbauer

Research output: Chapter in Book/Report/Conference proceedingChapter

14 Scopus citations

Abstract

The ability to create cell-derived decellularized matrices in a dish gives researchers the opportunity to possess a bioactive, biocompatible material made up of fibrillar proteins and other factors that recapitulates key features of the native structure and composition of in vivo microenvironments. By using cells in a culture system to provide a natural ECM, decellularization allows for a high degree of customization through the introduction of selected proteins and soluble factors. The culture system, culture medium, cell types, and physical environments can be varied to provide specialized ECMs for wide-ranging applications to study cell–ECM signaling, cell migration, cell differentiation, and tissue engineering purposes. This chapter describes a procedure for performing a detergent and high pH-based extraction that leaves the native, cell-assembled ECM intact while removing cellular materials. We address common evaluation methods for assessing the ECM and its composition as well as potential uses for a decellularized ECM.

Original languageEnglish (US)
Title of host publicationMethods in Cell Biology
PublisherAcademic Press Inc.
Pages97-114
Number of pages18
DOIs
StatePublished - 2018

Publication series

NameMethods in Cell Biology
Volume143
ISSN (Print)0091-679X

All Science Journal Classification (ASJC) codes

  • Cell Biology

Keywords

  • Cell–ECM interactions
  • Decellularization
  • Extracellular matrix
  • Scaffold
  • Tissue engineering

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  • Cite this

    Harris, G. M., Raitman, I., & Schwarzbauer, J. E. (2018). Cell-derived decellularized extracellular matrices. In Methods in Cell Biology (pp. 97-114). (Methods in Cell Biology; Vol. 143). Academic Press Inc.. https://doi.org/10.1016/bs.mcb.2017.08.007