TY - JOUR
T1 - Cell cycle-dependent regulation of yeast telomerase by Ku
AU - Fisher, Timothy S.
AU - Taggart, Andrew K.P.
AU - Zakian, Virginia A.
N1 - Funding Information:
We thank S. Dunaway, L. Goudsouzian, A. Ivessa, M. Sabourin, and L. Vega for critical reading of the manuscript. We thank A. Chan for technical assistance and D. Gottschling and A. Stellwagen for strains. This work was supported by US National Institutes of Health (NIH) grant GM43265. T.S.F. is a Leukemia and Lymphoma Society Research Fellow. A.K.P.T was supported in part by a postdoctoral fellowship from the Susan G. Komen Breast Cancer Foundation and also NIH grant T32 CA09528.
PY - 2004/12
Y1 - 2004/12
N2 - The heterodimeric Ku complex affects telomere structure in diverse organisms. We report here that in the absence of Ku, the catalytic subunit of telomerase, Est2p, was not telomere-associated in G1 phase, and its association in late S phase was decreased. The telomere association of Est1p, a telomerase component that binds telomeres only in late S phase, was also reduced in the absence of Ku. The effects of Ku on telomerase binding require a 48-nucleotide (nt) stem-loop region of TLC1 telomerase RNA. Ku interacts with TLC1 RNA via this 48-nt region throughout the cell cycle, but this interaction was reduced after telomere replication. These data support a model in which Ku recruits telomerase to telomeres in G1 phase when telomerase is inactive and promotes telomerase-mediated telomere lengthening in late S phase.
AB - The heterodimeric Ku complex affects telomere structure in diverse organisms. We report here that in the absence of Ku, the catalytic subunit of telomerase, Est2p, was not telomere-associated in G1 phase, and its association in late S phase was decreased. The telomere association of Est1p, a telomerase component that binds telomeres only in late S phase, was also reduced in the absence of Ku. The effects of Ku on telomerase binding require a 48-nucleotide (nt) stem-loop region of TLC1 telomerase RNA. Ku interacts with TLC1 RNA via this 48-nt region throughout the cell cycle, but this interaction was reduced after telomere replication. These data support a model in which Ku recruits telomerase to telomeres in G1 phase when telomerase is inactive and promotes telomerase-mediated telomere lengthening in late S phase.
UR - https://www.scopus.com/pages/publications/16544390953
UR - https://www.scopus.com/pages/publications/16544390953#tab=citedBy
U2 - 10.1038/nsmb854
DO - 10.1038/nsmb854
M3 - Article
C2 - 15531893
AN - SCOPUS:16544390953
SN - 1545-9993
VL - 11
SP - 1198
EP - 1205
JO - Nature Structural and Molecular Biology
JF - Nature Structural and Molecular Biology
IS - 12
ER -