Cell cycle-dependent regulation of yeast telomerase by Ku

Timothy S. Fisher, Andrew K.P. Taggart, Virginia A. Zakian

Research output: Contribution to journalArticle

168 Scopus citations

Abstract

The heterodimeric Ku complex affects telomere structure in diverse organisms. We report here that in the absence of Ku, the catalytic subunit of telomerase, Est2p, was not telomere-associated in G1 phase, and its association in late S phase was decreased. The telomere association of Est1p, a telomerase component that binds telomeres only in late S phase, was also reduced in the absence of Ku. The effects of Ku on telomerase binding require a 48-nucleotide (nt) stem-loop region of TLC1 telomerase RNA. Ku interacts with TLC1 RNA via this 48-nt region throughout the cell cycle, but this interaction was reduced after telomere replication. These data support a model in which Ku recruits telomerase to telomeres in G1 phase when telomerase is inactive and promotes telomerase-mediated telomere lengthening in late S phase.

Original languageEnglish (US)
Pages (from-to)1198-1205
Number of pages8
JournalNature Structural and Molecular Biology
Volume11
Issue number12
DOIs
StatePublished - Dec 2004

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

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