Cell-cell signaling by direct contact increases cell proliferation via a PI3K-dependent signal

Celeste M. Nelson, Christopher S. Chen

Research output: Contribution to journalArticle

199 Scopus citations

Abstract

We report a novel mechanism of cellular growth control. Increasing the density of endothelial or smooth muscle cells in culture increased cell-cell contact and decreased cell spreading, leading to growth arrest. Using a new method to independently control cell-cell contact and cell spreading, we found that introducing cell-cell contact positively regulates proliferation, but that contact-mediated proliferation can be masked by changes in cell spreading: Round cells with many contacts proliferated less than spread cells with none. Physically blocking cell-cell contact or inhibiting PI3K signaling abrogated cell-cell induced proliferation, but inhibiting diffusible paracrine signaling did not. Thus, direct cell-cell contact induces proliferation in these cells.

Original languageEnglish (US)
Pages (from-to)238-242
Number of pages5
JournalFEBS Letters
Volume514
Issue number2-3
DOIs
StatePublished - Mar 13 2002

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Keywords

  • Cell shape
  • Extracellular matrix
  • Intercellular adhesion
  • Microcontact printing

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