Capacitance cytometry: Measuring biological cells one by one

L. L. Sohn, O. A. Saleh, G. R. Facer, A. J. Beavis, R. S. Allan, D. A. Notterman

Research output: Contribution to journalArticle

175 Scopus citations

Abstract

Measuring the DNA content of eukaryotic cells is a fundamental task in biology and medicine. We have observed a linear relationship between the DNA content of eukaryotic cells and the change in capacitance that is evoked by the passage of individual cells across a 1-kHz electric field. This relationship is species-independent; consequently, we have developed a microfluidic technique - 'capacitance cytometry' - that can be used to quantify the DNA content of single eukaryotic cells and to analyze the cell-cycle kinetics of populations of cells. Comparisons with standard flow cytometry demonstrate the sensitivity of this new technique.

Original languageEnglish (US)
Pages (from-to)10687-10690
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume97
Issue number20
DOIs
StatePublished - Sep 26 2000

All Science Journal Classification (ASJC) codes

  • General

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