TY - JOUR
T1 - Caged luciferins for bioluminescent activity-based sensing
AU - Su, Timothy A.
AU - Bruemmer, Kevin J.
AU - Chang, Christopher J.
N1 - Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2019/12
Y1 - 2019/12
N2 - Bioluminescence imaging is a powerful modality for in vivo imaging owing to its low background and high signal-to-noise ratio. Because bioluminescent emission occurs only upon the catalytic reaction between the luciferase enzyme and its luciferin substrate, caging luciferins with analyte-reactive triggers offers a general approach for activity-based sensing of specific biochemical processes in living systems across cell, tissue, and animal models. In this review, we summarize recent efforts in the development of synthetic caged luciferins for tracking enzyme, small molecule, and metal ion activity and their contributions to physiological and pathological processes.
AB - Bioluminescence imaging is a powerful modality for in vivo imaging owing to its low background and high signal-to-noise ratio. Because bioluminescent emission occurs only upon the catalytic reaction between the luciferase enzyme and its luciferin substrate, caging luciferins with analyte-reactive triggers offers a general approach for activity-based sensing of specific biochemical processes in living systems across cell, tissue, and animal models. In this review, we summarize recent efforts in the development of synthetic caged luciferins for tracking enzyme, small molecule, and metal ion activity and their contributions to physiological and pathological processes.
UR - https://www.scopus.com/pages/publications/85067044160
UR - https://www.scopus.com/pages/publications/85067044160#tab=citedBy
U2 - 10.1016/j.copbio.2019.05.002
DO - 10.1016/j.copbio.2019.05.002
M3 - Review article
C2 - 31200275
AN - SCOPUS:85067044160
SN - 0958-1669
VL - 60
SP - 198
EP - 204
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
ER -