@inbook{8573d5ac0684465aaa8467f1fd90e406,
title = "Building Biomaterials to Mimic 3D Cell–Cell Junctions",
abstract = "Cell–cell interactions typically occur in a 3D context that is distinct from conventional 2D cell-substrate interactions in a Petri dish. Here, we describe a benchtop method to combine a 2D extracellular matrix surface with a 3D, vertical boundary functionalized with the extracellular domain of E-cadherin. The methodology is suitable for any biology laboratory without requiring advanced microfabrication equipment or training. Overall, this cell-mimetic interface uniquely recapitulates key aspects of cell–cell adhesion and can serve as a versatile, reductionist technique to study general cell–cell interactions in a 3D context.",
keywords = "Biomaterials, Cadherin, Cell–cell adhesion, Epithelia, Functionalization, Microfabrication, Scaffold",
author = "Cohen, {Daniel J.}",
note = "Publisher Copyright: {\textcopyright} The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024.",
year = "2024",
doi = "10.1007/978-1-0716-3854-5_6",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "101--112",
booktitle = "Methods in Molecular Biology",
}