Branching microtubule nucleation in xenopus egg extracts mediated by augmin and TPX2

Sabine Petry, Aaron C. Groen, Keisuke Ishihara, Timothy J. Mitchison, Ronald D. Vale

Research output: Contribution to journalArticlepeer-review

168 Scopus citations

Abstract

The microtubules that comprise mitotic spindles in animal cells are nucleated at centrosomes and by spindle assembly factors that are activated in the vicinity of chromatin. Indirect evidence has suggested that microtubules also might be nucleated from pre-existing microtubules throughout the spindle, but this process has not been observed directly. Here, we demonstrate microtubule nucleation from the sides of existing microtubules in meiotic Xenopus egg extracts. Daughter microtubules grow at a low branch angle and with the same polarity as mother filaments. Branching microtubule nucleation requires γ-tubulin and augmin and is stimulated by factors previously implicated in chromatin-stimulated nucleation, guanosine triphosphate(GTP)-bound Ran and its effector, TPX2. Because of the rapid amplification of microtubule numbers and the preservation of microtubule polarity, microtubule-dependent microtubule nucleation is well suited for spindle assembly and maintenance.

Original languageEnglish (US)
Pages (from-to)768-777
Number of pages10
JournalCell
Volume152
Issue number4
DOIs
StatePublished - Feb 14 2013

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

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