@article{212684d4e10b4192b11d3638e5245e90,
title = "bottleneck acts as a regulator of the microfilament network governing cellularization of the Drosophila embryo",
abstract = "A dynamic network of cortical microfilaments is associated with the cleavage furrow membranes during cellularization of the Drosophila embryo. A specific set of structural rearrangements in this network is required for orchestration and execution of its mechanistic roles. We describe the characterization of the gene bottleneck (bnk), mutations in which disturb the proper sequence of rearrangements of the microfilament network, leading to a variety of morphological defects during cellularization. bnk, whose expression is restricted to the blastoderm stages of Drosophila embryogenesis, encodes a novel, exceptionally basic protein that specifically colocalizes with the microfilament network. The expression pattern and mutant phenotype of bnk suggest a direct role for this element in regulation of the dynamic restructuring of the actin-based cytoskeleton of cellularizing Drosophila embryos.",
author = "Schejter, {Eyal D.} and Eric Wieschaus",
note = "Funding Information: We gratefully acknowledge the help and generosity of many colleagues, which enabled us to carry out our studies. We thank Peter Bryant, Jerry Manning, John Merriam, Alan Shearn, Larry Ztpursky, and the Bowling Green stock center for fly strains and phage DNA from the bnk region; Roger Karess for determining the cytology of the chp J1 rearrangement; Paul Ftsher for a-lamm, Manfred Frasch for c~-eve, and Erikur StemgPmsson and Judy Lengyel for a-tll antibodies; and Nick Brown, Marcus Noll, and John Bishop for phage hbraries. We thank Joe Goodhouse for expert instructton and advice on the use of the confocal mtcroscope, Ken I rvme, Marya Postner, Siegfried Roth, and Trudi Sch~ipbach for cntical reading of the manuscnpt, and all members of the Wieschaus and SchL~pbach labs for their constructive criticism, sound advice, and support throughout these studies. This work was supported by grant 5R01 HD15587 from the National Institutes of Health. E. D. S. was supported by postdoctoral fellowships from the Chaim Welzmann Fund and the New Jersey Commtssion on Cancer Research.",
year = "1993",
month = oct,
day = "22",
doi = "10.1016/0092-8674(93)80078-S",
language = "English (US)",
volume = "75",
pages = "373--385",
journal = "Cell",
issn = "0092-8674",
publisher = "Elsevier B.V.",
number = "2",
}