Both cyclin AΔ60 and BΔ97 are stable and arrest cells in M-phase, but only cycfin BΔ97 turns on cyclin destruction

Francis C. Luca, Ellen K. Shibuya, Cord E. Dohrmann, Joan V. Ruderman

Research output: Contribution to journalArticlepeer-review

182 Scopus citations

Abstract

Previous work has established that destruction of cyclin B is necessary for exit from mitosis and entry into the next interphase. Sea urchin cyclin B lacking an N-terminal domain is stable, permanently activates cdc2 kinase, resulting in mitotic arrest, and permanently activates the destruction pathway acting on full length cyclin B. Here we have compared the properties of clam cyclins A and B lacking related N-terminal domains. Both cyclin AΔ60 and BΔ97 bind to cdc2 kinase, keep it hyperactivated and block the completion of mitosis. By adding purified Acyclin proteins to a cell-free system at different cell cycle times, we find that when the cell-free system reaches the cyclin destruction point in the presence of either AΔ60 or BΔ97, the cyclin destruction pathway acting on full length cyclins fails to be turned off. However, the two cyclins differ dramatically in their ability to turn on cyclin destruction. When added to emetine-arrested interphase lysates devoid of endogenous cyclins, only cyclin BΔ97 activates the cyclin destruction system; cyclin AΔ60 does not. This functional difference between the two cyclin types, the first to be described, provides strong support for the idea that the two cyclins have different roles in the cell cycle and suggests that one specialized role of the cyclin B-cdc2 complex is to activate the cyclin destruction pathway and drive cells into interphase of the next cell cycle.

Original languageEnglish (US)
Pages (from-to)4311-4320
Number of pages10
JournalEMBO Journal
Volume10
Issue number13
StatePublished - 1991
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General Biochemistry, Genetics and Molecular Biology
  • General Immunology and Microbiology
  • Molecular Biology
  • General Neuroscience

Keywords

  • Cell cycle
  • Fertilization
  • Mitosis
  • Proteolysis
  • cdc2 kinase

Fingerprint

Dive into the research topics of 'Both cyclin AΔ60 and BΔ97 are stable and arrest cells in M-phase, but only cycfin BΔ97 turns on cyclin destruction'. Together they form a unique fingerprint.

Cite this