Block copolymer nanoparticles as nanobeads for the polymerase chain reaction

Siyan Zhang; Robert K. Prudhomme; A. James Link

doi:10.1021/nl200271d

Block copolymer nanoparticles as nanobeads for the polymerase chain reaction

Siyan Zhang, Robert K. Prudhomme, A. James Link

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

New sequencing technologies based on massively parallel signature sequencing (MPSS) have been developed to reduce the cost of genome sequencing. In some current MPSS platforms, DNA-modified micrometer-scale beads are used to template the polymerase chain reaction (PCR). Reducing the size of the beads to nanoscale can lead to significant improvements in sequencing throughput. To this end, we have assembled polymeric nanobeads that efficiently template PCR, resulting in DNA-decorated "nanobeads" with a high extent of functionalization.

Original language	English (US)
Pages (from-to)	1723-1726
Number of pages	4
Journal	Nano Letters
Volume	11
Issue number	4
DOIs	https://doi.org/10.1021/nl200271d
State	Published - Apr 13 2011

All Science Journal Classification (ASJC) codes

General Chemistry
Condensed Matter Physics
Mechanical Engineering
Bioengineering
General Materials Science

Keywords

Block copolymers
click chemistry
massively parallel signature sequencing
polymerase chain reaction
self-assembly

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10.1021/nl200271d

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AU - Prudhomme, Robert K.

AU - Link, A. James

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AB - New sequencing technologies based on massively parallel signature sequencing (MPSS) have been developed to reduce the cost of genome sequencing. In some current MPSS platforms, DNA-modified micrometer-scale beads are used to template the polymerase chain reaction (PCR). Reducing the size of the beads to nanoscale can lead to significant improvements in sequencing throughput. To this end, we have assembled polymeric nanobeads that efficiently template PCR, resulting in DNA-decorated "nanobeads" with a high extent of functionalization.

KW - Block copolymers

KW - click chemistry

KW - massively parallel signature sequencing

KW - polymerase chain reaction

KW - self-assembly

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Block copolymer nanoparticles as nanobeads for the polymerase chain reaction

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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