Bipolar cellular morphology of malignant melanoma in unstained human melanoma skin tissue

Kai Zhang; Wenkai Zhang; Chia Yi Yang; Haw Yang

doi:10.1117/1.3120491

Bipolar cellular morphology of malignant melanoma in unstained human melanoma skin tissue

Kai Zhang, Wenkai Zhang, Chia Yi Yang, Haw Yang

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Microstructures of unstained human melanoma skin tissues have been examined by multimodal nonlinear optical microscopy. The polarized shape of the individual melanoma cell can be readily recognized - a phenotype that has been identified in laboratory cultures as characteristic of proliferating melanocytes but has not been demonstrated in clinical instances. The results thus provide snapshots of invading melanoma cells in their native environment and suggest a practical means of connecting in vitro laboratory studies to in vivo processes.

Original language	English (US)
Article number	024042
Journal	Journal of Biomedical Optics
Volume	14
Issue number	2
DOIs	https://doi.org/10.1117/1.3120491
State	Published - 2009
Externally published	Yes

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomedical Engineering
Biomaterials

Keywords

cell-extracellular matrix interaction
optical microscopy
second harmonic generation (SHG)
skin cancer
two-photon excited fluorescence

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10.1117/1.3120491

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title = "Bipolar cellular morphology of malignant melanoma in unstained human melanoma skin tissue",

abstract = "Microstructures of unstained human melanoma skin tissues have been examined by multimodal nonlinear optical microscopy. The polarized shape of the individual melanoma cell can be readily recognized - a phenotype that has been identified in laboratory cultures as characteristic of proliferating melanocytes but has not been demonstrated in clinical instances. The results thus provide snapshots of invading melanoma cells in their native environment and suggest a practical means of connecting in vitro laboratory studies to in vivo processes.",

keywords = "cell-extracellular matrix interaction, optical microscopy, second harmonic generation (SHG), skin cancer, two-photon excited fluorescence",

author = "Kai Zhang and Wenkai Zhang and Yang, {Chia Yi} and Haw Yang",

note = "Funding Information: The authors thank Dr. S. E. Ruzin and Dr. D. Schichnes at the Biological Imaging Facility at UC Berkeley for their help in stained sample preparation and image acquisition. This work is supported in part by the University of California and Alfred P. Sloan Foundation, with special equipment supported by U.S. Department of Energy and by the the National Science Foundation.",

year = "2009",

doi = "10.1117/1.3120491",

language = "English (US)",

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journal = "Journal of Biomedical Optics",

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TY - JOUR

T1 - Bipolar cellular morphology of malignant melanoma in unstained human melanoma skin tissue

AU - Zhang, Kai

AU - Zhang, Wenkai

AU - Yang, Chia Yi

AU - Yang, Haw

N1 - Funding Information: The authors thank Dr. S. E. Ruzin and Dr. D. Schichnes at the Biological Imaging Facility at UC Berkeley for their help in stained sample preparation and image acquisition. This work is supported in part by the University of California and Alfred P. Sloan Foundation, with special equipment supported by U.S. Department of Energy and by the the National Science Foundation.

PY - 2009

Y1 - 2009

N2 - Microstructures of unstained human melanoma skin tissues have been examined by multimodal nonlinear optical microscopy. The polarized shape of the individual melanoma cell can be readily recognized - a phenotype that has been identified in laboratory cultures as characteristic of proliferating melanocytes but has not been demonstrated in clinical instances. The results thus provide snapshots of invading melanoma cells in their native environment and suggest a practical means of connecting in vitro laboratory studies to in vivo processes.

AB - Microstructures of unstained human melanoma skin tissues have been examined by multimodal nonlinear optical microscopy. The polarized shape of the individual melanoma cell can be readily recognized - a phenotype that has been identified in laboratory cultures as characteristic of proliferating melanocytes but has not been demonstrated in clinical instances. The results thus provide snapshots of invading melanoma cells in their native environment and suggest a practical means of connecting in vitro laboratory studies to in vivo processes.

KW - cell-extracellular matrix interaction

KW - optical microscopy

KW - second harmonic generation (SHG)

KW - skin cancer

KW - two-photon excited fluorescence

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ER -

Bipolar cellular morphology of malignant melanoma in unstained human melanoma skin tissue

Abstract

All Science Journal Classification (ASJC) codes

Keywords

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