Biophysical measurements of bacterial cell shape

Jeffrey P. Nguyen, Benjamin P. Bratton, Joshua W. Shaevitz

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

A bacteria’s shape plays a large role in determining its mechanism of motility, energy requirements, and ability to avoid predation. Although it is a major factor in cell fitness, little is known about how cell shape is determined or maintained. These problems are made worse by a lack of accurate methods to measure cell shape in vivo, as current methods do not account for blurring artifacts introduced by the microscope. Here, we introduce a method using 2D active surfaces and forward convolution with a measured point spread function to measure the 3D shape of different strains of E. coli from fluorescent images. Using this technique, we are also able to measure the distribution of fluorescent molecules, such as polymers, on the cell surface. This quantification of the surface geometry and fluorescence distribution allow for a more precise measure of 3D cell shape and is a useful tool for measuring protein localization and the mechanisms of bacterial shape control.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages227-245
Number of pages19
DOIs
StatePublished - Jun 1 2016

Publication series

NameMethods in Molecular Biology
Volume1440
ISSN (Print)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Keywords

  • 3D shape
  • Active contours
  • Bacteria
  • Cell shape
  • Deconvolution
  • Fluorescent microscopy
  • Point spread function

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    Nguyen, J. P., Bratton, B. P., & Shaevitz, J. W. (2016). Biophysical measurements of bacterial cell shape. In Methods in Molecular Biology (pp. 227-245). (Methods in Molecular Biology; Vol. 1440). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3676-2_17