Biological Applications of Protein Splicing

Miquel Vila-Perelló, Tom W. Muir

Research output: Contribution to journalReview articlepeer-review

134 Scopus citations


Protein splicing is a naturally occurring process in which a protein editor, called an intein, performs a molecular disappearing act by cutting itself out of a host protein in a traceless manner. In the two decades since its discovery, protein splicing has been harnessed for the development of several protein-engineering methods. Collectively, these technologies help bridge the fields of chemistry and biology, allowing hitherto impossible manipulations of protein covalent structure. These tools and their application are the subject of this Primer.

Original languageEnglish (US)
Pages (from-to)191-200
Number of pages10
Issue number2
StatePublished - Oct 15 2010
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • General Biochemistry, Genetics and Molecular Biology


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