TY - JOUR
T1 - Biological Applications of Protein Splicing
AU - Vila-Perelló, Miquel
AU - Muir, Tom W.
N1 - Funding Information:
We thank B. Fierz and N. Shah for valuable input. Some of the work discussed in this review was performed in the Muir laboratory and was supported by the NIH.
PY - 2010/10/15
Y1 - 2010/10/15
N2 - Protein splicing is a naturally occurring process in which a protein editor, called an intein, performs a molecular disappearing act by cutting itself out of a host protein in a traceless manner. In the two decades since its discovery, protein splicing has been harnessed for the development of several protein-engineering methods. Collectively, these technologies help bridge the fields of chemistry and biology, allowing hitherto impossible manipulations of protein covalent structure. These tools and their application are the subject of this Primer.
AB - Protein splicing is a naturally occurring process in which a protein editor, called an intein, performs a molecular disappearing act by cutting itself out of a host protein in a traceless manner. In the two decades since its discovery, protein splicing has been harnessed for the development of several protein-engineering methods. Collectively, these technologies help bridge the fields of chemistry and biology, allowing hitherto impossible manipulations of protein covalent structure. These tools and their application are the subject of this Primer.
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U2 - 10.1016/j.cell.2010.09.031
DO - 10.1016/j.cell.2010.09.031
M3 - Review article
C2 - 20946979
AN - SCOPUS:77957758860
SN - 0092-8674
VL - 143
SP - 191
EP - 200
JO - Cell
JF - Cell
IS - 2
ER -